- ￥5000
- GeneTex
- 美国
- GTX32289
- 2025年07月13日
- WB, ICC/IF
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of PAK4. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX32289
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody is raised against PAK4 phosphorylated at Ser474. Based on sequence homology, it is predicted to react with PAK5 and PAK6 when phosphorylated at the corresponding residues - Ser602/Ser560.
- 抗体英文名：
PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody
- 抗体名：
PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) 抗体
- 规格：
100 μl
ICC/IF analysis of formalin-fixed Raw264.7 cells using GTX32289 PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
WB analysis of EGF-treated HEK293T (A), EGF-treated Raw264.7 (B), EGF-treated PC12 (C) whole cell lysates using GTX32289 PAK4 (phospho Ser474) / PAK5 (phospho Ser602) / PAK6 (phospho Ser560) antibody.
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文献和实验Ramos-Alvarez I et al., Am J Physiol Gastrointest Liver Physiol 2018 (PMID:29672153)
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
下调不能说明活性降低，因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外，还要做磷酸化的GSK3-beta的蛋白表达。 （2）应该活性形式和非活性形式都做。一般来讲，GSK3-beta在Ser9位点磷酸化之后活性收到抑制，而在216位点磷酸化之后，其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了，另外还要同时检测GSK3-beta的总蛋白表达，这样才能全面的说明问题。
Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
spectrometry data is providing evidence that almost every protein in the cell undergoes some form of post?translational modification. We describe a protocol to use the scan?x Web site to view predicted acetylation sites in the human proteome and predicted
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