- ￥5600
- GeneTex
- 美国
- GTX25189
- 2025年07月15日
- WB, ICC/IF
- Rabbit
- Human, Mouse, Cat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human integrin b1 receptor that contains threonines 788 and 789 (based on Swiss Protein database, accession number P05558).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Cat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX25189
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Integrin beta 1 / CD29 (phospho Thr788/Thr789)
- 抗体英文名：
Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody
- 抗体名：
Integrin beta 1 / CD29 (phospho Thr788/Thr789) 抗体
- 规格：
50 μl
ICC/IF analysis of THP-1 cells treated with 100ng/mL of IFN-gamma for 15 minutes using GTX25189 Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% PFA
Permeabilization : 0.1% Triton X-100 for 10 minutes
WB analysis of serum-starved mitotic HeLa cells generated by treatment with 100 ng/mL taxol for 16 hours using GTX25189 Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
WB analysis of membrane enriched extracts (30 μg lysate) of HeLa (Lane 1) treated with Paclitaxel (Lane 2), HepG2 (Lane 3) treated with Paclitaxel (Lane 4), and 10 μL of conditioned media from HepG2 cell line (Lane 5) using GTX25189 Integrin beta 1 / CD29 (phospho Thr788/Thr789) antibody.
Dilution : 1:250
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文献和实验Qin N et al., Eur J Med Chem 2016 (PMID:27598237)
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
4 ). 2. Other antibodies used were b-catenin (phospho-Thr41/Ser37/Ser33) (#9561; Cell Signaling Technology), b-catenin (#9562;Cell Signaling Technology), CRMP2 (pT514/509) (#PB-043; Kinasource, Dundee, Scotland) and CRMP2 (#AB-042;Kinasource). 3. Blocking buffer
序列，靠近外侧N端的3个或4个重复序列中含有Asp-X-Asp-X-Asp-Gly-X-X-Asp或类似结构，与integrin分子结合二价阳离子（Mg2+ ）有关，并与β亚单位共同构成粘合素分子的配体结合部位，其中α亚单位的二价阳离子结合区与 integrin分子配体结合的特异性和亲和力有关。某些integrin分子的α亚单位在转录后被剪接为两段，一段为劳作膜部分，较小，约20～30kDa；另一段为胞膜外部分，较大，两者通过二硫键连接起来（图2-1）。电镜下可见integrin分子有一个球状头部，向下
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