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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Extracellular domain of human EGF-receptor from head and neck carcinoma.
- 亚型:
IgG2a
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX20231
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rat
- 应用范围:
IHC-P, IHC-Fr, FACS, IP
- 浓度:
1.0 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody, clone ICR10 binds to epitope B from EGFR
- 抗体英文名:
EGFR antibody [ICR10]
- 抗体名:
EGFR 抗体 [ICR10]
- 规格:
100 μg
FACS analysis of A431 cells using GTX20231 EGFR antibody [ICR10].
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文献和实验Aurelien Bidaud-Meynard et al., bioRxiv 2018 (Epub)
研究中[8]。(3)MAB528与MAB225MAB528、MAB225是针对EGFR胞外区的鼠源性抗体[9]。它们与EGF、TGFα和EGFR有相似的亲和力,竞争性与EGFR配体结合区结合,减低酪氨酸激酶的活化。能明显抑制培养细胞及裸鼠肿瘤移植物的增殖。(4)其它EMD55900[10]、 ICR62[11]是鼠源性单克隆抗体。在头颈部上皮性肿瘤体外试验显示了抗增生活性。CIBCNSH3[11]也是针对过表达EGFR肿瘤的单克隆抗体。体外实验CIBCNSH3与过表达EGFR细胞共育,发现98%细胞
RUVKUN Antibody Staining Protocol revised 10/29/90
RUVKUN Antibody Staining Protocol revised 10/29/90 Fixation: 1. C. elegans N2 grown in liquid or plates. Harvest worms. Wash for 2 hours at room temp. in 1X M9 or PBS. 2. Fix in 5 ml of FRESH THAT DAY 1% paraformaldehyde:
the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes.(15) Wash with 50 ml 10 mM Tris 7.5 and then wash with 50 ml 10 mM Tris 7.5/500 mM NaCl. Elute with 5 ml 100 mM Glycine pH 2.5 into 1 ml 1M Tris 8.0.(16
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