Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Monoclonal
标记物:
Unconjugated
适应物种:
Human, Mouse, Rat, Primate
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX15731
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Mouse
应用范围:
WB, ICC/IF, IHC-P, IP, ELISA, ChIP assay, IHC
浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
CREB
抗体英文名:
CREB antibody [LB9]
抗体名:
CREB 抗体 [LB9]
规格:
100 μg
IHC-P analysis of human bladder and rectal tissue using GTX15731 CREB antibody [LB9]. Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Dilution : 1:200
ICC/IF analysis of HeLa cells using GTX15731 CREB antibody [LB9]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:500 for at least 1 hour at room temperature
ICC/IF analysis of HeLa cells using GTX15731 CREB antibody [LB9]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:400 for at least 1 hour at roomtemperature
ChIP analysis of 2 million HeLa cells using GTX15731 CREB antibody [LB9]. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to promoter of NR4A3, CYR61 gene used as positive control target, and the MYOD, SAT2 used as negative control target.
WB analysis of nuclear extracts (30 μg lysate) of Jurkat (Lane 1), HeLa (Lane 2), K-562 (Lane 3), Hep G2 (Lane 4), NIH/3T3 (Lane 5), C2C12 (Lane 6), U-2 OS (Lane 7), A549 (Lane 8), MDA-MB-231 (Lane 9) and T98G (Lane10) using GTX15731 CREB antibody [LB9]. Dilution : 1:500
WB analysis of 25 ug of various whole cell lysates using GTX15731 CREB antibody [LB9]. Dilution : 1:500
IHC-P analysis of human tonsil tissue using GTX15731 CREB antibody [LB9]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
ICC/IF analysis of MDA-MB-231 cells using GTX15731 CREB antibody [LB9]. Panel e shows the no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% PFA Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 5 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature