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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human alpha Internexin. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX130053
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IP
- 浓度:
0.66 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
alpha Internexin
- 抗体英文名:
alpha Internexin antibody
- 抗体名:
alpha Internexin 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Immunoprecipitation of alpha Internexin protein from U87-MG whole cell extracts using 5 μg of alpha Internexin antibody (GTX130053).
Western blot analysis was performed using alpha Internexin antibody (GTX130053) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted by 1:1000.
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted at 1:1000.
alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted by 1:1000.
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted at 1:1000.
alpha Internexin antibody detects alpha Internexin protein expression by immunofluorescent analysis.
Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% PFA at RT for 15 min.
Red: alpha Internexin protein stained by alpha Internexin antibody (GTX130053) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
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文献和实验Gama Sosa MA et al., Acta Neuropathol Commun 2019 (PMID:30626447)
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
Immunohistochemistry / Immunocytochemistry Procedures
of fixative for each antigen. However frozen sections give less morphological detail and resolution than other methods. Rabbit anti-alpha-internexin (Cat No. AB5354
marrow. The prime suspect: an adhesion molecule called alpha2 integrin. The molecule showed increased expression on memory cells, and its ligand is predominantly expressed in bone marrow tissue. Also, when the researchers blocked alpha2 integrin
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