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Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys4 of human Histone H3. The exact sequence is proprietary.
IgG
Liquid
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
Polyclonal
Unconjugated
Human, Mouse, Rat, Zebrafish, Drosophila
12 months from the shipping date of the product.
Human
GTX128954
Primary Antibodies
Available
GeneTex
Rabbit
WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, Dot, ChIP assay, EM
0.08 mg/ml(Please refer to the vial label for the specific concentration.)
Histone H3K4me3 (Tri-methyl Lys4)
Histone H3K4me3 (Tri-methyl Lys4) antibody
Histone H3K4me3 (Tri-methyl Lys4) 抗体
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H3K4me3 (Tri-methyl Lys4) antibody detects Histone H3K4me3 (Tri-methyl Lys4) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3K4me3 (Tri-methyl Lys4) stained by Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-Histone H3K4me3 (trimethyl Lys4) antibody. The precipitated DNA was detected by PCR with primer set targeting to GADPH gene.
Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Dotblot analysis of anti-Histone H3 (tri-Methyl Lys4) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (tri-Methyl Lys4) antibody (GTX128954) 1:5000 dilution.
A: Peptide samples of H3K4 un
B: Peptide samples of H3K4me1
C: Peptide samples of H3K4me2
D: Peptide samples of H3K4me3
E: Peptide samples of H3K4ac
Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: PFA-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:100.
HeLa whole cell and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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