- ￥1700 - 4000
- GeneTex
- 美国
- GTX128954
- 2025年07月13日
- WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, Dot, ChIP assay, EM
- Rabbit
- Human, Mouse, Rat, Zebrafish, Drosophila
企业认证
相关产品推荐更多 >
![PTP1B antibody [4F8F11]](https://custom.dxycdn.com/trademd/upload/pic/2013/11/12/A1383891150.jpg!small.t.1)
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys4 of human Histone H3. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Zebrafish, Drosophila
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX128954
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, Dot, ChIP assay, EM
- 浓度：
0.08 mg/ml(Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H3K4me3 (Tri-methyl Lys4)
- 抗体英文名：
Histone H3K4me3 (Tri-methyl Lys4) antibody
- 抗体名：
Histone H3K4me3 (Tri-methyl Lys4) 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Histone H3K4me3 (Tri-methyl Lys4) antibody detects Histone H3K4me3 (Tri-methyl Lys4) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Histone H3K4me3 (Tri-methyl Lys4) stained by Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse esophagus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse esophagus.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
ChIP was performed with HeLa chromatin extract and 5 μg of either normal rabbit IgG or anti-Histone H3K4me3 (trimethyl Lys4) antibody. The precipitated DNA was detected by PCR with primer set targeting to GADPH gene.
Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Dotblot analysis of anti-Histone H3 (tri-Methyl Lys4) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (tri-Methyl Lys4) antibody (GTX128954) 1:5000 dilution.
A: Peptide samples of H3K4 un
B: Peptide samples of H3K4me1
C: Peptide samples of H3K4me2
D: Peptide samples of H3K4me3
E: Peptide samples of H3K4ac
Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: PFA-fixed 2 day-post-fertilization zebrafish embryo.
Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:100.
HeLa whole cell and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验Laurent C et al., Blood 2019 (PMID:31774495)
Di Giorgio E et al., Nucleic Acids Res 2020 (PMID:31754707)
Meng M et al., Immunogenetics 2019 (PMID:31297569)
Di Giorgio E et al., PLoS Genet 2017 (PMID:28419090)
Eid W et al., Biol Chem 2016 (PMID:27343473)
Pang L et al., Biosci Rep 2021 (PMID:34724040)
Chiu YF et al., Sci Rep 2020 (PMID:32427884)
Methyl DNA Immunoprecipitation
can be done. This methylation analysis is highly specific due to the use of a well-characterized monoclonal antibody and each IP is directly quality controlled: two essential keys for reliable results. In addition, the kit protocol is fast and user-friendly. The METHYL kit
Histone lysine and arginine methylation involved in gene activation and silencing is dynamically regulated. However, partly limited to the research technologies previously available, the dynamics of global histone methylation on a site
In Vitro Histone Demethylase Assays
methyltransferases and the histone demethylases. Two families of histone demethylases have been identified to remove methyl groups from lysine side chain through different reaction mechanisms in presence of distinct cofactors. Amine oxidase LSD1 family requires
技术资料暂无技术资料 索取技术资料
