- ￥1700 - 4000
- GeneTex
- 美国
- GTX124113
- 2025年07月15日
- WB, IHC-P, Dot
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding asymmetric di-methyl Arg17 of human Histone H3. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX124113
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, IHC-P, Dot
- 浓度：
0.62 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H3R17me2 (Asymmetric Di-methyl Arg17)
- 抗体英文名：
Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody
- 抗体名：
Histone H3R17me2 (Asymmetric Di-methyl Arg17) 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Histone3 (di-methyl Arg17) antibody detects HIST1H3A protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
15 % SDS-PAGE
Histone3 (di-methyl Arg17) antibody (GTX124113) dilution: 1:1000
Histone H3R17me2 (dimethyl Arg17) antibody detects Histone H3R17me2 (dimethyl Arg17) protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H3R17me2 (dimethyl Arg17) antibody (GTX124113) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone3 (di-methyl Arg17) antibody detects HIST1H3A protein at nucleus on AGS xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded AGS xenograft.
Histone3 (di-methyl Arg17) antibody (GTX124113) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Histone H3R17me2 (dimethyl Arg17) antibody detects Histone H3R17me2 (dimethyl Arg17) protein at nucleus on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
Histone H3R17me2 (dimethyl Arg17) antibody (GTX124113) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Dotblot analysis of anti-Histone3 (di-methyl Arg17) antibody with peptide samples.
Peptide samples (0.1 μg) were spotted onto positively charged nylon membrane and blotted with Histone3 (di-methyl Arg17) antibody (GTX124113) at different dilution as indicated.
A: Peptide samples of Histone3 (di-methyl Arg17)
B: Peptide samples of Histone H3.1
Histone H3R17me2 (dimethyl Arg17) antibody detects Histone H3R17me2 (dimethyl Arg17) protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Histone H3R17me2 (dimethyl Arg17) antibody (GTX124113) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Zhou Y et al., Oncol Lett 2020 (PMID:33101492)
Methyl DNA Immunoprecipitation
can be done. This methylation analysis is highly specific due to the use of a well-characterized monoclonal antibody and each IP is directly quality controlled: two essential keys for reliable results. In addition, the kit protocol is fast and user-friendly. The METHYL kit
组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白；在细胞正常 pH 值时，组蛋白带有正电荷，这样它们就可以和 DNA 结合，这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实，组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg ， Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白： H1 ， H2A ， H2B ， H3
Histone lysine and arginine methylation involved in gene activation and silencing is dynamically regulated. However, partly limited to the research technologies previously available, the dynamics of global histone methylation on a site
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