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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human Lipoic acid synthetase. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX115528
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Lipoic acid synthetase
- 抗体英文名:
Lipoic acid synthetase antibody [N1C2]
- 抗体名:
Lipoic acid synthetase 抗体 [N1C2]
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX115528 diluted at 1:2000
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文献和实验Xu L et al., PLoS One 2016 (PMID:27706190)
. Measurements of acyl?CoA ligase activity include monitoring the rate of appearance of AMP or PPi, or the CoA adduct. N?acyltransferases catalyze formation of an amino acid conjugate from the CoA?activated intermediate, releasing CoA. This reaction is monitored
. The use of graphitized carbon column instead of classical C18 reversed-phase material is shown to be well suited to detect low abundant glycoforms and to provide in one shot information regarding both the oligosaccharide structure and the amino acid
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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