- ￥1700 - 4000
- GeneTex
- 美国
- GTX109832
- 2025年07月14日
- WB, ICC/IF, IHC-P
- Rabbit
- Human, Mouse, Rat, Drosophila
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 1A. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Drosophila
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX109832
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
0.73 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
alpha Tubulin 1A
- 抗体英文名：
alpha Tubulin 1A antibody
- 抗体名：
alpha Tubulin 1A 抗体
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 μg Jurkat whole cell extract
B. 30 μg Raji whole cell extract
C. 30 μg 293T whole cell extract
D. 30 μg A431 whole cell extract
E. 30 μg HeLa whole cell extract
F. 30 μg HepG2 whole cell extract
G. 30 μg H1299 whole cell extract
H. 30 μg HCT116 whole cell extract
I. 30 μg MCF-7 whole cell extract
J. 30 μg NT2D1 whole cell extract
K. 30 μg PC-3 whole cell extract
L. 30 μg U87-MG whole cell extract
10% SDS-PAGE
alpha Tubulin 1A antibody (GTX109832) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using alpha Tubulin 1A(GTX109832) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: alpha Tubulin 1A stained by alpha Tubulin 1A antibody (GTX109832) diluted at 1:500.
Blue: Hoechst 33342 staining.
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 μg drosophila lysate/extract
10% SDS-PAGE
alpha Tubulin 1A antibody (GTX109832) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell extract
B. 30 μg mouse brain extract
10% SDS-PAGE
alpha Tubulin 1A antibody (GTX109832) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin 1A antibody (GTX109832) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 μg rat brain extract
10% SDS-PAGE
alpha Tubulin 1A antibody (GTX109832) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Tubulin 1A antibody detects alpha Tubulin 1A protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: alpha Tubulin 1A stained by alpha Tubulin 1A antibody (GTX109832) diluted at 1:500.
Blue: Hoechst 33342 staining.
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文献和实验Wei-Chou Lin et al., Am J Cancer Res 2015;5(11)
Chen PH et al., Oncogene 2012 (PMID:21996739)
Lin WC et al., Cancer Res 2010 (PMID:20587516)
Yi-Chang Wang et al., Nature Communication 2018 9()
Chiu JH et al., J Transl Med 2017 (PMID:28472954)
Immunofluorescent Localization of Tubulin
antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film
. Here, we describe the detection of autoAbs to various self-antigens with different tissue restriction patterns which includes collagens, k-α1 tubulin, vimentin, and myosin. We also discuss their relevance in monitoring for rejection following solid organ transplantation.
Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms
Alpha tubulin comprises a C-terminal tyrosine residue, which is subject to cyclic removal from the peptide chain by a still uncharacterized carboxypeptidase and re-addition to the chain by a tubulin tyrosine ligase. We have shown in different
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