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    研选SOX2 antibody [N1C3]

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    SOX2 antibody [N1C3]

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    • 商家诚信度 
    • 入驻年限  7年
    • 2456 Alton PkwyIrvine, CA 92606 USA
      • 货号:

        gtx101507

      • 应用范围:

        WB,ICC/IF,IHC-P,IHC-Fr,FACS,IP

      • 适应物种:

        Human,Mouse,Rat

      • 宿主:

        Rabbit

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse esophagus.
      SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

      Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

      Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      *The competitor is not affiliated with GeneTex and does not endorse this product.

      Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      Flow cytometry on human embryonic stem cells, staining with SOX2 (GTX101507)antibody at 1:100 dilution(purple) or rabbit IgG (black).

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.
      Sample: Frozen sectioned adult mouse retina.
      Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
      Red: Protein kinase C alpha staining.
      Blue: Fluoroshield with DAPI (GTX30920).

      Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(GTX101507) antibody at 1:100 dilution.
      Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.
      Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: SOX2 stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.
      Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
      Scale bar= 10 μm.

      SOX2 antibody detects SOX2 protein by western blot analysis. Mouse ESC lysate (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507) or SOX2 antibody [GT1876] (GTX627404).
      Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

      Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis.
      Sample: Frozen-sectioned adult mouse hippocampus.
      Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
      Blue: Fluoroshield with DAPI (GTX30920).

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.
      Sample: Frozen sectioned E13.5 rat brain.
      Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
      Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
      Blue: Fluoroshield with DAPI (GTX30920).

      Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(GTX101507) antibody at 1:160 dilution.

      Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507).
      Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

      SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500.

      Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(GTX101507) antibody at 1:80 dilution.

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis.
      Sample: Paraffin-embedded rat brain stem.
      SOX2 antibody [N1C3] (GTX101507) dilution: 1:500.

      Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse fetal brain by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse fetal brain.
      Green: SOX2 antibody [N1C3] (GTX101507) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
      Blue: Nuclear staining with Hoechst 33342.

      Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse fore brain.
      SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

      Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human esophageal carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human esophageal carcinoma.
      SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

      Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human cervical carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human cervical carcinoma.
      SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

      Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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