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    研选SOX2 antibody [N1C3]

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    SOX2 antibody [N1C3]

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      • 货号:

        gtx101507

      • 应用范围:

        WB,ICC/IF,IHC-P,IHC-Fr,FACS,IP

      • 适应物种:

        Human,Mouse,Rat

      • 宿主:

        Rabbit

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse esophagus.
      SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

      Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

      Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      *The competitor is not affiliated with GeneTex and does not endorse this product.

      SOX2 antibody detects SOX2 protein by western blot analysis. Mouse ESC lysate (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507) or SOX2 antibody [GT1876] (GTX627404).
      Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

      Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

      SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis.
      Sample: Frozen-sectioned adult mouse hippocampus.
      Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
      Blue: Fluoroshield with DAPI (GTX30920).

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.
      Sample: Frozen sectioned E13.5 rat brain.
      Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
      Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
      Blue: Fluoroshield with DAPI (GTX30920).

      Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(GTX101507) antibody at 1:160 dilution.

      Flow cytometry on human embryonic stem cells, staining with SOX2 (GTX101507)antibody at 1:100 dilution(purple) or rabbit IgG (black).

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.
      Sample: Frozen sectioned adult mouse retina.
      Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.
      Red: Protein kinase C alpha staining.
      Blue: Fluoroshield with DAPI (GTX30920).

      Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(GTX101507) antibody at 1:100 dilution.
      Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.
      Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: SOX2 stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.
      Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
      Scale bar= 10 μm.

      Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507).
      Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

      SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500.

      Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(GTX101507) antibody at 1:80 dilution.

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis.
      Sample: Paraffin-embedded rat brain stem.
      SOX2 antibody [N1C3] (GTX101507) dilution: 1:500.

      Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse fetal brain by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse fetal brain.
      Green: SOX2 antibody [N1C3] (GTX101507) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
      Blue: Nuclear staining with Hoechst 33342.

      Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse fore brain.
      SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

      Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human esophageal carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human esophageal carcinoma.
      SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

      Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

      SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human cervical carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human cervical carcinoma.
      SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

      Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

      Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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