Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse
保质期:
12 months from the shipping date of the product.
抗原来源:
Human
目录编号:
GTX100622
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, ICC/IF, FACS, IP
浓度:
0.32 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
OCT4
抗体英文名:
Oct4 antibody
抗体名:
Oct4 抗体
规格:
100 μl/25 μl
规格:
100 μl
产品价格:
¥4000.0
规格:
25 μl
产品价格:
¥1700.0
Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX100622) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX100622) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX100622) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX100622) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX100622)antibody at 1:50 dilution(green) or rabbit IgG (black).
Immunofluorescence analysis of PFA-fixed human embryonic stem cell, using Oct4(GTX100622) antibody at 1:200 dilution.
Sample (20 μg of whole cell lysate) A: mouse ESC 12% SDS PAGE GTX100622 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% PFA at RT for 15 min.Green: Oct4 stained by Oct4 antibody (GTX100622) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 μm.
Immunofluorescence analysis of PFA-fixed Human ESC, using Oct4(GTX100622) antibody at 1:400 dilution.
Immunofluorescence analysis of PFA-fixed human embryonic stem cell, using Oct4(GTX100622) antibody at 1:100 dilution.