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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human ALDH2. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Zebrafish
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX101429
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IHC-Wm, ELISA, IHC, Sandwich ELISA
- 浓度:
0.23 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
ALDH2
- 抗体英文名:
ALDH2 antibody
- 抗体名:
ALDH2 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
ALDH2 antibody detects ALDH2 protein at mitochondria on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: PFA-fixed zebrafish embryos.
ALDH2 antibody (GTX101429) dilution: 1:200.
ALDH2 antibody detects ALDH2 protein by Western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) at a dilution of 1:2000.
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ALDH2 antibody (GTX101429) at 1:300 dilution.
ALDH2 antibody detects ALDH2 protein at mitochondria on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
ALDH2 antibody (GTX101429) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
ALDH2 antibody detects ALDH2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) at a dilution of 1:500.
HepG2 whole cell and cytoplasm extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) diluted at 1:500.
ALDH2 antibody detects ALDH2 protein by western blot analysis.
A. 30 μg GL261 whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysae/extract
D. 30 μg Raw264.7 whole cell lysate/extract
7.5% SDS-PAGE
ALDH2 antibody (GTX101429) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ALDH2 antibody detects ALDH2 protein at mitochondria by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% PFA at RT for 15 min.
Green: ALDH2 protein stained by ALDH2 antibody (GTX101429) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
ALDH2 antibody detects ALDH2 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ALDH2 antibody (GTX101429) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
ELISA detection of ALDH2 using GTX101429 for capture at a concentration of 2.5 μg/mL and GTX88073 for detection at a concentration of 1.5 μg/mL.
Immunohistochemical analysis of paraffin-embedded AGS xenograft, using ALDH2(GTX101429) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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文献和实验Liu S et al., Hepatology 2012 (PMID:22031168)
Masato A et al., NPJ Parkinsons Dis 2023 (PMID:36966140)
Shiau-Mei Chen et al., Biomolecules 2021 (PMID:34680107)
Kitakaze T et al., Arch Biochem Biophys 2020 (PMID:32151565)
Gueugneau M et al., BMC Genomics 2014 (PMID:25532418)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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