- 询价
- Cell Signaling Technology
- 9852
- USA
- 2026年01月17日
- 参见网站
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- 详细信息
- 文献和实验
- 技术资料
- 宿主：
参见网站
- 供应商：
赛信通（上海）生物试剂有限公司暨美国CST中国分公司
- 库存：
大量
- 抗原来源：
参见网站
- 是否单克隆：
多克隆
- 规格：
5 ml
产品说明书：http://media.cellsignal.com/pdf/9852.pdf
Description
Chaps Cell Extract Buffer can be used to lyse cells under nondenaturing conditions and is recommended for the preparation of cytoplasmic cell lysates to be used with our caspase signaling pathway antibodies (see Companion Products).
裂解细胞提取缓冲液可以在非变性的条件下裂解细胞，建议应用于准备细胞浆裂解物，联合使用我们的半胱氨酸信号转导通路抗体（见相关产品）。
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 9491 Cleaved Caspase-7 (Asp198) Antibody
- 9492 Caspase-7 Antibody
- 9501 Cleaved Caspase-9 (Asp330) Antibody (Human Specific)
- 9502 Caspase-9 Antibody (Human Specific)
- 9661 Cleaved Caspase-3 (Asp175) Antibody
- 9662 Caspase-3 Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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文献和实验Preparation of nuclear extract and cytoplasmic extract
PMSFBuffer D (Dialysis Buffer): For 2 L20 mM HEPES,pH 7.9 1 M HEPES 7.9 40 ml20 % Glycerol Glycerol 400 ml100 mM KCl3 M KCl66.67 ml0.2 mM EDTA 0.5 M EDTA 800 µl0.5 mM DTT 1M DTT 1 ml0.2 mM PMSF 0.2 M PMSF 2 mlSplicing Extract Preparation1.Resuspend cell
Small Scale Yeast Whole Cell Extract for IP
beads. Assay protein concentration using BioRad or Pierce assays. Freeze extracts and store at -80 deg. Expect 10-15 mg/ml protein. Extract Buffer: 100 mM Tris pH 7.9 250 mM Ammonium Sulfate 1 mM EDTA 10% Glycerol
Yeast mini whole cell extract using glass beads for western analysis
----------------------- Extraction Buffer : 200 mM Tris pH 8.0 150 mM Ammonium sulfate 10% glycerol 1 mM EDTA Add fresh on day of extract preparation : 2 mM DTT PMSF Benzamidine Leupeptin Pepstatin Chymostatin Protease inhibitors 0.1 M PMSF (100x) 16 mg/ml Ethanol
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