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- boshi
- CAS号：78432-77-6
- 2025年07月12日
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文献和实验Steady State ATPase Assays Coupled Enzyme System
Add taxol to 10 µM (final concentration in assay = 6.5 µM). 6. For each assay, add to cuvette in the following order: DDW = 19.5 µL - (volume of motor + MTs) 130 µL Tris-MgCl2 -DTT
Tubulin Polymerization with GTP/GMPCPP/Taxol
BRB80 (1X): 80 mM PIPES, 1 mM MgCl2 , 1 mM EGTA, pH 6.8 with KOH (generally made as a 5X stock and stored at 4¡C) 100 mM GTP 100 mM GMPCPP Taxol : 10 mM stock; 100 µM, 10 µM and 1 µM dilutions
Preparation of Segmented and Polarity Marked Microtubules
made as a 5X stock and stored at 4¡C) 100 mM GTP 100 mM GMPCPP Taxol : 10 mM stock; 200 µM, 20 µM and 2 µM dilution all in anhydrous DMSO; sold under tradename "Paclitaxel" by Sigma Bright GMPCPP Seed Mix (2 mg/ml; 1 part rhodamine tubulin
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