Our Automated SDS-MW Analysis Chemistries is designed for the separation and sizing of protein-SDS complexes using a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 kDa to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes.
This chemistry can also be used to effectively quantify the amount of protein and to determine the purity of a protein product.
SDS-MW Analysis Chemistries Includes:
Separation Capillary, 57 cm x 50µm ID bare fused silica, 2 pieces
SDS-Gel Separation Buffer, 140 mL
SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL
SDS Protein Sizing Standard (10 to 225 kDa) 16 mg/mL, 100 µL
Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL