- ¥950
- Clontech
- clontech
- 630466
- 2025年09月28日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 技术资料
- 英文名：
Aureobasidin A
- 库存：
现货
- 供应商：
上海伟进科技有限公司
- 规格：
1mg
Aureobasidin A for Yeast Two-Hybrid Studies
Aureobasidin A (AbA) is a cyclic depsipeptide antibiotic, isolated from the filamentous fungusAureobasidium pullulans R106, which is toxic to yeast at low concentrations (0.1–0.5 µg/ml). Sensitive species include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida glabrata, Aspergillus nidulans, and A. niger.
AbA inhibits a yeast enzyme, inositol phosphorylceramide (IPC) synthase, which is expressed from the AUR1 gene (1, 2). Expression of a mutant gene, AUR1-C, in transformed yeast confers resistance to the drug. It is this gene that is used in Matchmaker Gold systems as a reporter for protein interactions.
Perfect Reporter for Yeast Two-Hybrid Studies
AbA selection virtually eliminates the high numbers of background colonies that often plague low-stringency primary screens that use nutritional markers alone (e.g. HIS3). Because AbA actually kills sensitive yeast cells, rather than merely retarding their growth, AbA-based selection greatly favors the growth and identification of genuinely positive clones. In general practice, a high percentage of clones that emerge from low-stringency primary screens using AbA selection alone, are subsequently verified on high-stringency secondary screens that select for all four Matchmaker Gold reporters (AUR1-C,HIS3, ADE2 and MEL1).
Features
Applications
Properties
References
|
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
技术资料暂无技术资料 索取技术资料
