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- Sino Biological
- 10202-V02H1
- 2026年01月26日
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- 规格：
100 µg
蛋白名称：CMV Glycoprotein B蛋白, CMV Glycoprotein B protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907) of human CMV gB (AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') precursor was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 80 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to bind biotinylated Human CD209-Fc (Cat:10200-H01H) in functional ELISA.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Asn 25
蛋白分子量：The recombinant human CMV glycoprotein B/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 1045 amino acids and has a predicted molecular mass of 118 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of gB/Fc monomer is approximately 160-170 kDa due to glycosylation.
蛋白NP号：AAA45920.1
蛋白氨基酸序列：Met1-Lys700 & Arg777-Val907
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Applications for a Dual Fluorescent Human Cytomegalovirus in the Analysis of Viral Entry
a dual fluorescent HCMV, where the capsid-associated tegument protein pp150 is labelled with EGFP and the envelope glycoprotein gM with mCherry. This dual labelled virus allows for the separate tracking of the viral envelope fusing with a cellular
辛杭体结合的配体，因而非特异性反应很低。抗地高辛的特异性抗体与脊椎动物甾体激素的交叉反应不到1％，若此抗体的Fc部分通过蛋白酶水解的方法除去后，则可完全排除细胞Fc受体非特异性的吸附作用。本方法可以用于福尔马林固定的石蜡包埋的组织切片、冰冻切片和培养的或从组织中分离的细胞凋亡测定。 一、试剂配制 1、磷酸缓冲液PBS（pH7.4）：磷酸钠盐 50mM，NaCl 200mM。 2、蛋白酶K（200μg/ml，pH7.4）：蛋白酶K 0.02g；PBS 100ml。 3、含2%H2
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
), nuclear gene encoding mitochondrial protein, mRNA 2307 NM_001654 Homo sapiens v-raf murine sarcoma 3611 viral oncogene homolog 1 (ARAF1), mRNA 846 NM_002967 Homo sapiens scaffold attachment factor B (SAFB ), mRNA 545 NM_001183 Homo sapiens ATPase
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