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文献和实验: rate of migration will depend on the strength of their net surface charges: :: The protein that carries more +ve charges
CO-IMMUNOPRECIPITATION ANALYSIS OF PROTEIN-PROTEIN INTERACTIONS
CO-IMMUNOPRECIPITATION ANALYSIS OF PROTEIN-PROTEIN INTERACTIONS This protocol uses total cell extracts to analyze putative protein-protein interactions in eukaryotic cells. One can also use nuclear extracts as a source
Isolation of Protein from Tissue
Place the tissues on labeled aluminum foil and immediately place in dry ice.It is imperative that the tissues stay cold so that protease do not have time to act on the protein. Place the tissues in a round bottom tube and add Brij buffer
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