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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
ADA
- 保质期：
1年
- 保存条件：
2-8°C
- 库存：
100⁺瓶
- 供应商：
上海睿安生物19121878899
- 规格：
2ml/支
Roche货号10102105001腺苷脱氨酶(ADA)上海睿安生物Merck-Sigma-Millipore-Roche官方正式授权代理商19121878899
腺苷脱氨酶(ADA)
from calf intestine
别名:ADA
属性
产品名称:腺苷脱氨酶(ADA),from calf intestine
biological source:bovine(calf) intestine
form:solution
specific activity:~200 units/mg protein(At 25°C with adenosine as the substrate.)
mol wt:
230-440 kDa(high)
30-47 kDa(low)
packaging:pkg of 2m(10mg)
manufacturer/tradename:Roche
optimum pH:7.0-7.4
shipped in:wet ice
storage temp:2-8°C
Quality Level:100
说明
Application
腺苷脱氨酶已被用于预处理原代库普弗细胞。
腺苷脱氨酶(ADA)已被用作处理巨噬细胞用无血清培养基(SFM)的一种成分。
Biochem/physiol Actions
腺苷脱氨酶(ADA)参与腺苷脱氨为肌苷和2′脱氧腺苷脱氨基成为 2′-脱氧肌苷的过程。
腺苷脱氨酶(ADA)可催化腺苷类似物脱氨基，形成相应的肌苷类似物。它在结肠直肠癌中的浓度很高，并且是一种强效的结核性胸膜炎标志物。ADA缺乏会影响T-和B-淋巴细胞，从而引起严重联合免疫缺陷(SCID)，最终导致淋巴细胞减少症。
Analysis Note
污染物:碱性磷酸酶、AMP-脱氨酶、鸟嘌呤酶和核苷磷酸化酶，每种均<0.01%
General description
腺苷脱氨酶(ADA)是一种酶，主要存在于近端小肠和十二指肠中。它也被称为腺苷氨基水解酶。它是一种锌金属酶，具有大分子量(230−440 kDa)和小分子量(30−47 kDa)同工型。
腺苷脱氨酶(ADA)是一种锌金属酶。
Other Notes:仅用于生命科学研究。不用于诊断操作。
Physical form:50％甘油(v/v)溶液，含有10mM磷酸钾，pH约6
Preparation Note:储存条件(工作溶液):ADA(所有制剂)可以在2至8℃下对20mM磷酸盐缓冲液(pH7)透析而不损失活性。透析的制剂在-15至-25℃下稳定。
同行评审论文(部分:文献)
Adenosine deaminase deficiency: a review
Flinn AM and Gennery AR
Orphanet Journal of Rare Diseases, 13(1), 65-65 (2018)
Pdx-1 is required for activation in vivo from a duodenum-specific enhancer
Dusing MR, et al.
The Journal of biological chemistry, 276(17), 14434-14442 (2001)
Purification and characterization of intestinal adenosine deaminase from mice
Singh L and Sharma R
Molecular and Cellular Biochemistry, 204(1-2), 127-134 (2000)
Development of adenosine deaminase-specific IgY antibodies: Diagnostic and inhibitory application
Lupicka-Slowik A, et al.
Applied Biochemistry and Biotechnology, 184(4), 1358-1374 (2018)
Optimal protocol for the differentiation and metabolic analysis of human adipose stromal cells.
Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65(2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be
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- 作者
- 内容
- 询问日期
文献和实验该产品被引用文献
Roche货号10102105001腺苷脱氨酶(ADA)上海睿安生物Merck-Sigma-Millipore-Roche官方正式授权代理商19121878899
腺苷脱氨酶(ADA)
from calf intestine
别名:ADA
属性
产品名称:腺苷脱氨酶(ADA),from calf intestine
biological source:bovine(calf) intestine
form:solution
specific activity:~200 units/mg protein(At 25°C with adenosine as the substrate.)
mol wt:
230-440 kDa(high)
30-47 kDa(low)
packaging:pkg of 2m(10mg)
manufacturer/tradename:Roche
optimum pH:7.0-7.4
shipped in:wet ice
storage temp:2-8°C
Quality Level:100
说明
Application
腺苷脱氨酶已被用于预处理原代库普弗细胞。
腺苷脱氨酶(ADA)已被用作处理巨噬细胞用无血清培养基(SFM)的一种成分。
Biochem/physiol Actions
腺苷脱氨酶(ADA)参与腺苷脱氨为肌苷和2′脱氧腺苷脱氨基成为 2′-脱氧肌苷的过程。
腺苷脱氨酶(ADA)可催化腺苷类似物脱氨基，形成相应的肌苷类似物。它在结肠直肠癌中的浓度很高，并且是一种强效的结核性胸膜炎标志物。ADA缺乏会影响T-和B-淋巴细胞，从而引起严重联合免疫缺陷(SCID)，最终导致淋巴细胞减少症。
Analysis Note
污染物:碱性磷酸酶、AMP-脱氨酶、鸟嘌呤酶和核苷磷酸化酶，每种均<0.01%
General description
腺苷脱氨酶(ADA)是一种酶，主要存在于近端小肠和十二指肠中。它也被称为腺苷氨基水解酶。它是一种锌金属酶，具有大分子量(230−440 kDa)和小分子量(30−47 kDa)同工型。
腺苷脱氨酶(ADA)是一种锌金属酶。
Other Notes:仅用于生命科学研究。不用于诊断操作。
Physical form:50％甘油(v/v)溶液，含有10mM磷酸钾，pH约6
Preparation Note:储存条件(工作溶液):ADA(所有制剂)可以在2至8℃下对20mM磷酸盐缓冲液(pH7)透析而不损失活性。透析的制剂在-15至-25℃下稳定。
同行评审论文(部分:文献)
Adenosine deaminase deficiency: a review
Flinn AM and Gennery AR
Orphanet Journal of Rare Diseases, 13(1), 65-65 (2018)
Pdx-1 is required for activation in vivo from a duodenum-specific enhancer
Dusing MR, et al.
The Journal of biological chemistry, 276(17), 14434-14442 (2001)
Purification and characterization of intestinal adenosine deaminase from mice
Singh L and Sharma R
Molecular and Cellular Biochemistry, 204(1-2), 127-134 (2000)
Development of adenosine deaminase-specific IgY antibodies: Diagnostic and inhibitory application
Lupicka-Slowik A, et al.
Applied Biochemistry and Biotechnology, 184(4), 1358-1374 (2018)
Optimal protocol for the differentiation and metabolic analysis of human adipose stromal cells.
Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65(2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be
文献支持
Roche货号10102105001腺苷脱氨酶(ADA)上海睿安生物Merck-Sigma-Millipore-Roche官方正式授权代理商19121878899
￥3669.08
