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Apogee血液微颗粒分析仪
Apogee血液微颗粒分析仪能检测小至70nm小颗粒的流式细胞仪
微生物、细胞外囊泡、外泌体、大病毒、纳米颗粒、动物细胞、人类细胞等
市面上大部分流式细胞仪主要针对细胞而设计,散射光的检测极限通常是500nm附近,检测500nm以下的生物颗粒通常依靠荧光探针,可是有时不能完全依靠荧光标记来区分不同细胞。这是多方面的原因决定的,如非特异性结合,细胞碎片的存在,结块的抗体或者荧光染料的摄取低下等,因此只有结合散射光才能得到理想的结果。;然而很多生物颗粒如细胞外囊泡,直径一般在300nm以下,比细胞小得多,产生的散射光信号非常微弱,故难以被传统流式细胞仪检测到。Apogee Flow Systems的Apogee血液微颗粒分析仪具有非常好的散射光和荧光灵敏度和分辨率,可检测从细胞到小至70nm的小颗粒,为亚微米生物颗(100nm-1000nm)粒检测带来了新希望!
70nm灵敏度/10nm分辨率 Apogee血液微颗粒分析仪在亚微米生物颗粒检测领域中独领风骚
·Apogee血液微颗粒分析仪拥有70nm散射光灵敏度
·非常适合微生物,细胞外囊泡,纳米颗粒&大型病毒检测
·同样适用于常规流式应用,不仅限于微小颗粒
Apogee血液微颗粒分析仪应用
细胞微粒(Microparticles)和外泌体(Exosomes)
·2个或3个光散射角度范围带来极高的灵敏度
·微泡可用于第二代诊断
·基于血液的癌症诊断检测
·血管生物学和血栓形成研究
·治疗药物给药
血小板
A50-Micro的高灵敏度非常适合血小板应用:
·血小板反应性
·血小板聚集
·循环活化血小板
·血小板微颗粒
·钙流
·细菌污染
微生物/病毒(如:巨细胞病毒)
·各种样品中细菌的快速检测
·细胞周期分析和遗传学
·细胞活性
·荧光蛋白
·病毒学
动物/人类细胞
常规流式分析:细胞凋亡/活力、线粒体膜电位分析、植物倍体分析、DNA含量分析等
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- 作者
- 内容
- 询问日期
文献和实验Brain, Behavior, and Immunity
Volume 123, January 2025, Pages 422-441
View Paper
Zhucheng Lin, Xuan Luo, Jason R. Wickman, Deepa Reddy, Jason T. DaCunza, Richa Pande, Yuzhen Tian, Ezgi E. Kasimoglu, Vivian Triana, Jingyun Lee, Cristina M. Furdui, Desmond Pink, Ahmet Sacan, Seena K. Ajit
Inflammatory pain resolution by mouse serum-derived small extracellular vesicles
2025
Alzheimer's Dement. 2024;1-13
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Dayarathna T, Roseborough AD, Gomes J, et al.
Nanoscale flow cytometry-based quantification of blood-based extracellular vesicle biomarkers distinguishes MCI and Alzheimer's disease
2024
Cytometry. 2023;103(8):670-83
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Pink D, Basu A, Wong M, Pham D, Valencia J, Triana V, et al.
Antibody titrations are critical for microflow cytometric analysis of extracellular vesicles
2023
Neurobiology of Disease
Volume 177, February 2023, 106001
View paper
A.D. Roseborough, Y. Zhu, L. Zhao, S.R. Laviolette, S.H. Pasternak, S.N. Whitehead
Fibrinogen primes the microglial NLRP3 inflammasome and propagates pro-inflammatory signaling via extracellular vesicles: Implications for blood-brain barrier dysfunction
2023
Journal of Neuroinflammation
View paper
A.D.Roseborough, S.J.Myers, R.Khazaee, Y. Zhu, L.Zhao, E.Iorio, F.M.Elahi, S. H.Pasternak and S.N.Whitehead
Plasma derived extracellular vesicle biomarkers of microglia activation in an experimental stroke model
2023
Neurobiology of Disease 177 (2023) 106001
View paper
A.D. Roseborough, Y. Zhu, L. Zhao, S.R. Laviolette, S.H. Pasternak, S.N. Whitehead
Fibrinogen primes the microglial NLRP3 inflammasome and propagates pro-inflammatory signaling via extracellular vesicles: Implications for blood-brain barrier dysfunction
2023
ACS Earth Space Chem
View paper
Serge Nader, Alexandre Baccouche, Fiona Connolly, Maya Abou-Ghanem, Sarah A. Styler, John D. Lewis, Desmond Pink, and Sheref S. Mansy
Model Atmospheric Aerosols Convert to Vesicles upon Entry into Aqueous Solution
2022
Mol Oncol. 2022 Dec 15
DOI: 10.1002/1878-0261.13362
View paper
Robert J Paproski , Desmond Pink , Deborah L Sosnowski , Catalina Vasquez, John D Lewis
Building predictive disease models using extracellular vesicle microscale flow cytometry and machine learning
2022
PubMed PMID: 36408626
DOI: 10.1042/BSR20222185
View paper
Roberto Villalobos-Labra , Ricky Liu , Floor Spaans , Tamara Saez , Anita Quon, Michael Wong , Desmond Pink , John Lewis , Manu Vatish , Sandra T Davidge, Christy-Lynn M Cooke
Placenta-Derived Extracellular Vesicles from Preeclamptic and Healthy Pregnancies Impair ex vivo Vascular Endothelial Function
2022
Nanoscale, 2022, 14, 9781
Yohan Kim, Edwin van der Pol, Ali Arafa, Ishwor Thapa, Cameron J. Britton, Jorgena Kosti, Siyang Song, Vidhu B. Joshi, Ree M. Erickson, Hesham Alie, Fabrice Lucien
Calibration and standardization of extracellular vesicle measurements by flow cytometry for translational prostate cancer research
2022
Biomedicines 2021, 9, 124.
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Jaco Botha, Haley R. Pugsley, Aase Handberg
Conventional, High-Resolution and Imaging Flow Cytometry: Benchmarking Performance in Characterisation of Extracellular Vesicles
2021
Platelets31.2 (2020): 206-214.
Rosinska, Justyna, et al.
"Effect of acetylsalicylic acid intake on platelet derived microvesicles in healthy subjects."
2020
PLoS ONE 15(8)
Palviainen M, Saraswat M, Varga Z, Kitka D, Neuvonen M, Puhka M, et al.
Extracellular vesicles from human plasma and serum are carriers of extravesicular cargo. Implications for biomarker discovery.
2020
Cells, 2020
J Karkowska-Kuleta, K Kulig, E Karnas, E Zuba-Surma
Characteristics of Extracellular Vesicles Released by the Pathogenic Yeast-Like Fungi Candida glabrata, Candida parapsilosis and Candida tropicalis
2020
bioRxiv (2020).
Cooper, Tyler T., et al.
"Ultrafiltration Segregates Tissue Regenerative Stimuli Harboured Within and Independent of Extracellular Vesicles."
2020
Gastroenterology (2020)
Dasgupta D et al.
IRE1A Stimulates Hepatocyte-derived Extracellular Vesicles That Promote Inflammation in Mice With Steatohepatitis
2020
Front. Oncol., 04 June 2020
L. G. Rikkert et al.
Cancer-ID: Toward Identification of Cancer by Tumor-Derived Extracellular Vesicles in Blood.
2020
Brain. 2020 May 1;143(5):1476-1497.
Min Guo et al.
Microglial Exosomes Facilitate synuclein Transmission in Parkinson's Disease.
2020
PLoS ONE 15(6): e0233443.
Rikkert LG, de Rond L, van Dam A, van Leeuwen TG, Coumans FAW, de Reijke TM, et al.(2020)
Detection of extracellular vesicles in plasma and urine of prostate cancer patients by flow cytometry and surface plasmon resonance imaging.
2020
Platelets, 2020, 31(1): 26-32.
Gasecka A, Nieuwland R, Budnik M, et al.
Randomized controlled trial protocol to investigate the antiplatelet therapy effect on extracellular vesicles (AFFECT EV) in acute myocardial infarction[J].
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bioRxiv, 2020
Maia J, Batista S, Couto N, et al.
Population Analysis of Extracellular Vesicles in Microvolumes of Biofluids[J].
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Neuro-Oncology, Volume 22, Issue 7, July 2020, Pages 967-978
Benjamin T. Himes et al.
The role of extracellular vesicles and PD-L1 in glioblastoma-mediated immunosuppressive monocyte induction.
2020
Mitochondrion 8 August 2020
Olivia R.Stephens et al.
Characterization and origins of cell-free mitochondria in healthy murine and human blood.
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Cell Death Dis 11, 677 (2020).
Gidlof, O., Bader, K., Celik, S. et al.
Inhibition of the long non-coding RNA NEAT1 protects cardiomyocytes from hypoxia in vitro via decreased pri-miRNA processing.
2020
BMC Ophthalmology volume 20, Article number: 233 (2020)
Zhong-Fang Yuan et al.
MicroRNA-182-5p protects human lens epithelial cells against oxidative stress-induced apoptosis by inhibiting NOX4 and p38 MAPK signalling.
2020
Colloids and Surfaces B: Biointerfaces (2020)
Hou Y-chen, Li J-an, Zhu S-jie, Cao C, Tang J-nan, Zhang J-ying, Guan S-kang
Tailoring of cardiovascular stent material surface by immobilizing exosomes for better pro-endothelialization function
2020
View paper
Desmond Pink, Michael Wong, Diana Pham, Renjith Pillai , Leanne Stifanyk, Sylvia Koch, Rebecca Hiebert, Oliver Kenyon, John Lewis
Performance Qualification for Micro Flow Cytometers: Understanding technical limitations to improve your research
Jul-20
THE JOURNAL OF UROLOGY 1 Apr 2019
Fabrice Lucien-Matteoni et al.
NEXT-GENERATION LIQUID BIOPSIES USING EXTRACELLULAR VESICLE DETECTION BY NANOSCALE FLOW CYTOMETRY.
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Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis[J].
2019
Journal of diabetes research, 2019
Hohendorff J, Drozdz A, Borys S, et al.
Effects of Negative Pressure Wound Therapy on Levels of Angiopoetin-2 and Other Selected Circulating Signaling Molecules in Patients with Diabetic Foot Ulcer[J].
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。Apogee A50-Micro是市场上唯一能够通过散射光检测小至100nm小颗粒的流式细胞仪,在细胞外囊泡的检测上优于任何一个竞争对手。 Figure 1. Technological improvement in forward scatter (FS) for microparticle (MP) measurement: (A) Beads resolution
,将在一块小的芯片上花很少量样品和试剂以很短的时间同时完成大量实验;在分析化学领域,它可以使以前大的分析仪器变成平方厘米尺寸规模的分析仪,将大大节约资源和能源。芯片实验室由于排污很少,所以也是一种“绿色”技术。二、 芯片实验室的发展历史与国内现状芯片实验室或称微全分析系统是由瑞士Ciba-Geigy公司的Manz与Widmer在1990年提出。他们最初的想法是发展一种可能作为一个化学分析所需的全部部件和操作集成在一起的微型器件,强调“微”与“全”。所以把µ-TAS看作是化学分析仪器的微型化。1993
。芯片实验室由于排污很少,所以也是一种“绿色”技术。中国SciEi.com. 二、 芯片实验室的发展历史与国内现状 芯片实验室或称微全分析系统是由瑞士Ciba-Geigy公司的Manz与Widmer[2]在1990年提出。他们最初的想法是发展一种可能作为一个化学分析所需的全部部件和操作集 成在一起的微型器件,强调“微”与“全”。所以把µ-TAS看作是化学分析仪器的微型化。1993年Harrison和 Manz等人在平板微芯片上实现了毛细管电泳与流动注射分 析,借电渗流实现
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