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联硕生物
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Axygen® 0.5 mL polypropylene thin wall PCR tubes with flat caps are designed to fit standard 96 well blocks and are available in an assortment of colors. Ultra-thin and consistent wall thickness allows precise thermal transfer and even insertion cap ensures a tight fit to prevent evaporation.
(This is the suggested replacement for PCR-05-X)
RNase-/DNase-free
Nonpyrogenic
Nonsterile
Manufactured with 99.9% pure virgin polypropylene
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文献和实验Chromosome Conformation Capture (3C) (PROT05)
C and add desired amount of T4 DNA ligase. Incubate for 4 hours (see note 4 ). DNA Purification Cross-links are reversed and the DNA is purified prior to PCR analysis. Add proteinase K (100µg/ml final) and incubate at 65°C overnight
The In Situ Detection of PCR-Amplified Hepatitis C RNA
to the obligatory tissue destruction required for DNA extraction. This problem has been circumvented by the field of in situ PCR. This chapter will describe the theoretical basis of in situ PCR using hepatitis C as a model system. The goals of this manuscript
Detection of the Hepatitis C Virus by RT-PCR
The hepatitis C virus (HCV) is an orgainsm of the age of molecular biology, for its discovery and much of the research into the infection have relied heavily on molecular techniques. The development of molecular cloning enabled a successful











