DSMZ no:ACC 2
Species: murine (mouse) (Mus musculus)
Cell type: connective tissue fibroblast
Origin: established from the normal subcutaneous areolar and adipose tissue of a male C3H/An mouse; used as target in TNF detection assays
Biosafety level: 1
Permissions and restrictions: A
DSMZ Cell Culture Data: Morphology: fibroblasts growing as monolayer
Medium: 90% RPMI 1640 + 10% h.i. FBS
Subculture: split confluent cultures 1:5 to 1:10 using trypsin (do not use trypsin/EDTA); after 2-3 days monolayer will be confluent; split 2-3 times a week; seed out initially at about 1.0-2.0 x 106 cells/25 cm2 Incubation: at 37 °C with 5% CO2 Doubling time: ca. 21-24 hours
Harvest: ca. 4-8 x 106 cells/25 cm2 Storage: frozen with 70% medium, 20% FBS, 10% DMSO