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文献和实验Replication timing by density transfer
whether the digest was complete. I generally blot and probe the gel anyway. CsCl gradients 9. Mix each sample (90-92 microliters, restriction enzyme and all) with 9.141 g of CsCl solution, to give a refractive index of 1.4042-1.4043. (Remember, it's a lot
In Situ HYBRIDIZATION TO TISSUE SECTIONS
shape facilitates later separation of sections, one from another. Mount the block onto a wooden specimen holder using melted wax (just melt the shavings from the trimmed block with a hot spatula and quickly press the block into the pool of melted
Selection of a Platform for Mutation Detection
is most suited for their particular needs. A practical guide is provided in this unit, including a brief overview, information on assay parameters, design and cost considerations, as well as platform flexibility and scalability of the assay. Although the focus
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