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小动物电子测痛仪也叫做电子触觉测试仪,用于对大鼠、小鼠足部进行轻触,来测量老鼠的机械痛阈值或触觉阈值。
小动物电子测痛仪是专为大鼠、小鼠机械疼痛研究设计的精密仪器,集成了高灵敏度传感器与数据采集管理软件,为神经科学、药理学及疼痛病理学研究提供精确、可重复的疼痛评估手段,助力科学家深入探索疼痛机制及开发新型镇痛疗法。
用于测试触觉灵敏度的经典仪器是Semmes-Weinstein纤毛机械刺激丝套装。但是Semmes-Weinstein纤毛机械刺激丝的方法非常耗时耗力,一个测试实验需要使用多个不同纤维丝进行多次和反复足底刺激。电子触觉测试仪可用来替代传统的Semmes-Weinstein(Von Frey Hairs)纤毛机械刺激针,可直接测得动物的机械痛阈和触觉阈测量,不需要进行反复的测试和繁杂的计算。设备轻巧易用,测量精确。
老鼠感觉到爪子被刺激时,会做出缩足反应。电子触觉测试仪使用测试探针对老鼠足底进行触觉刺激,力度由小到大逐渐增加力度,通过判断老鼠的缩足反应,来采集和分析老鼠的触觉阈值。
电子触觉测试仪相比传统方法的优势特点:
· 使用电子触觉测试仪进行一个测试实验,只需要进行一次足底刺激即可得到准确的结果;
· 数据可直接用于统计分析,能够节省很多的精力和时间;
· 测量精度高,测试力度能够读取到1000g以内的具体数值,精度可达0.1g;
· 不需要进行繁杂的统计和计算;
· 使用一个标准直径的刚性探针进行测试,能够避免不同力度纤维丝具有不同的直径而产生的触觉误差;
· 能够避免因为反复多次的测试对动物耐受力的影响,减小测试误差;
型号:38450
产品特点:
· 测试探针被固定在测试手柄上,通过测试探针自下而上对老鼠足底的刺激,老鼠会在刺激达到最大耐受力度时抬脚躲避,此时可通过脚踏开关来判断测试结果;
· 测试主机会显示和记录测试结果,包括最大测试力度和动物反应时间;
· 实验人员可根据本次测试老鼠的活动情况,接受或者拒绝本次测试结果;
· 被接受的测试结果会记录在主机中,可传到电脑进行统计;
· 为了方便测试,测试手柄端部采用了棱镜式设计,方便进行定位和瞄准;
· 设备中包含有助于稳定探针加力速率的软件工具;
主要参数:
· 最大适用力度:1000g
· 分辨率:0.1g
· 自动记录动物反应
· 辅助用户控制探针的加力速率
· 采用棱镜设计,有助于定位目标
电子触觉测试仪中使用的金属探针与经典的动态足底测试仪 Aesthe-siometer 37450 中使用的金属探针相同,可以对两种仪器的结果进行一致性比较。
带棱镜式设计的手柄,在进行“接触式刺激”
大鼠和小鼠测试探针细节
小鼠测试探针细节
测试手柄的主要特色:
具备有效的峰值检测器,自动捕捉和记录测试过程中施加的最大力度值;
具备可靠的自动检测动物反应功能;
具备辅助稳定探针加力速率的功能;
数据监测和存储:
测试主机具有存储和查看功能;
可将数据导出到电脑进行数据统计和分析;
可导出Excel或文本格式;
软件中可查看施加的力(红线)、所需的目标力率(蓝线)和峰值检测
基本配置:
带棱镜和刺激传感器的测试手柄,测试主机,脚踏板、软件和USB电缆。
选配:
测试支架



大鼠、小鼠测试笼
参考文献:
【1】Pain Relieving and Neuroprotective Effects of Non-opioid Compound, DDD-028, in the Rat Model of Paclitaxel-Induced Neuropathy
L Micheli, R Rajagopalan, E Lucarini, A Toti, C Parisio… - …, 2021 - Springer
【2】Dexpramipexole blocks Nav1. 8 sodium channels and provides analgesia in multiple nociceptive and neuropathic pain models
M Urru, M Muzzi, E Coppi, G Ranieri, D Buonvicino… - Pain, 2020 - journals.lww.com
【3】The Anti-Arthritic Efficacy of Khellin Loaded in Ascorbyl Decanoate Nanovesicles after an Intra-Articular Administration
G Vanti, L Di Cesare Mannelli, L Micheli, L Cinci… - Pharmaceutics, 2021 - mdpi.com
【4】Key role of gut microbiota in anhedonia-like phenotype in rodents with neuropathic pain
C Yang, X Fang, G Zhan, N Huang, S Li, J Bi… - Translational …, 2019 - nature.com
【5】Ameliorative effect of imperatorin in chemically induced fibromyalgia: role of NMDA/NFkB mediated downstream signaling
A Kaur, L Singh, N Singh, MS Bhatti, R Bhatti - Biochemical pharmacology, 2019 - Elsevier



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文献和实验[HTML] Fine‐touch pressure thresholds in the adult penis
ML Sorrells, JL Snyder, MD Reiss, C Eden… - BJU …, 2007 - Wiley Online Library
... The fine-touch sensitivity of 19 locations on the penis was measured using Semmes-Weinstein
monofilament touch-test sensory evaluators (North Coast Medical Supply, Morgan Hill, CA,
USA) [15–17] to create a penile sensitivity map (Figs 1 and 2). image ...
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[HTML] An experimental paradigm for the prediction of Post-Operative Pain (PPOP)
R Landau, JC Kraft, LY Flint, B Carvalho… - JoVE (Journal of …, 2010 - jove.com
... Pathway - Pain and Sensory Evaluation System, Medoc Advanced Medical Systems, B–kel 14L
Hot Tub, Boekel Scientific, 180g Touch-Test Sensory Evaluator, North Coast Medical, Morgan
Hill, California, Electronic von Frey, Bioseb, Cedex, France, References. ...
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Auditory grip force feedback in the treatment of writer's cramp
B Baur, W Fürholzer, C Marquardt… - Journal of Hand Therapy, 2009 - Elsevier
... The average age was 54.3 (±8.0) years. The mean duration of WC was 12.6 ± 16.9 years (range
0.5–48 yr). Touch sensibility measured by two-point discrimination for all patients was within
the normal range (mean: 2.6 ± 0.5 mm; Touch-Test, North Coast Medical, Inc.). Table 1. ...
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... A lower-extremity sensory examination was conducted using a 10-g Semmes-Weinstein
monofilament (Touch-Test Sensory Evaluator, North Coast Medical Inc, Morgan Hill, California)
and vibration perception threshold testing (VPT Tester, Salix Medical, San Antonio, Texas ...
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JG Modir, MS Wallace - Analgesia: Methods and Protocols, 2010 - Springer
... 4. Touch-Test Sensory Filament 5.46 (North Coast Medical, Morgan Hill, CA). 5. 26-g von Frey
hair (Semmes-Weinstein monofilaments, Stoelting, IL). 6. ... 3. Touch-Test Sensory Filament 5.46
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C LoPinto, WB Young, A Ashkenazi - Cephalalgia, 2006 - Wiley Online Library
... allodynia (BA) was tested by gently applying a folded 4 × 4-in gauze pad to the skin, 10 times
at a frequency of 2/s as we previously described (7). Pressure allodynia (PA) was tested by
applying von Frey hairs (VFH) (Touch-Test™ Sensory Evaluators; North Coast Medical, Inc. ...
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A Akbari, H Moodi, F Ghiasi… - Journal of …, 2007 - search.proquest.com
... We assessed sensory status using a biothesiometer (Bio-Medical Instrument Company, Newbury,
Ohio) and by applying a Semmes-Weinstein 10 g monofilament (Touch-Test Sensory Evaluator,
North Coast Medical, Inc, Morgan Hill, California) to the plantar surface of the foot. ...
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T Ogawa, T Suzuki, N Oishi, X Zhang, I Naert, K Sasaki - Odontology, 2011 - Springer
... The TDT of each tooth was measured by calibrated monofilaments (Touch-Test ® ,
North Coast Medical, CA, USA) with 20 different diameters corresponding to 20 target
forces (0.008–300 g) [10]. Tactile stimulation was applied ...
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Antinociceptive effect of CNS peroxynitrite scavenger in a mouse model of orofacial pain
JF Yeo, SF Ling, N Tang, WY Ong - Experimental brain research, 2008 - Springer
... The carrageenan injected area of the face was probed with a von Frey hair of 4.08 log units
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Stability and morbidity of Le Fort I osteotomy with bioresorbable fixation: a randomized controlled trial
LK Cheung, IHS Yip, RLK Chow - … journal of oral and maxillofacial surgery, 2008 - Elsevier
... The static light touch test was performed using a Touch-Test Sensory Evaluator kit
(NC12775, North Coast Medical Inc., CA, USA). Individual filaments of ascending
diameters were pressed against the infra-orbital skin until they bent. ...
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Acidic buffer induced muscle pain evokes referred pain and mechanical hyperalgesia in humans
LAF Law, KA Sluka, T McMullen, J Lee… - Pain, 2008 - Elsevier
... Cutaneous sensation was measured at the same six sensory test locations using
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perception threshold testing (VPT Tester; Xilas Medical Inc, San Antonio, Texas).17 ...
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... To diagnose neuropathy, each test subject underwent testing with a 5.07/10 g Semmes-Weinstein
monofilament test (SWMT) (Touch-Test; North Coast Medical, Morgan Hill, CA), biothesiometer
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Disease management for the diabetic foot: effectiveness of a diabetic foot prevention program to reduce amputations and hospitalizations
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... We identified sensory neuropathy from evaluation with a 10 gm Semmes-Weinstein
monofilament (Touch-Test Sensory Evaluator, North Coast Medical Inc., Morgan Hill,
CA) and Biothesiometer-VPT Meter (Xilas Medical, San Antonio, TX). ...
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,所以水合氯醛逐渐被淘汰。为什么水合氯醛不适用于小动物麻醉呢?其实主要原因刚才已经说了,因为它只能催眠不能镇痛。只要阻断中枢神经就能产生催眠作用,这个非常容易。而哺乳动物的痛觉感受器通常是游离的神经末梢,属于化学感受器,一旦遇到伤害性刺激,细胞就释放 K+、H+、5-羟色胺、前列腺素等物质激活痛觉感受器。大家知道,如果手被烫了一下会先缩回手,然后大脑才会反应过来刚才自己被烫了,就是这个道理。显然,水合氯醛只作用于中枢神经系统,是无法阻断痛觉感受器达到镇痛效果的。2有同学可能会继续问,只要大脑感觉不到疼痛
测定的时间也大致一样,如第一次在上午测定,以后均应在上午测定。(6)防止有大便阻塞和动物挣扎造成直肠损伤及出血现象。(7)测定时尽可能使动物处于自然状态,勿使其过于紧张、恐惧。三、脉搏的检查方法检查犬、猫、兔等较大动物的脉搏时,先将动物略加固定,待其安静后,用右手伸入动物股部内侧,用手指按股动脉测脉率1分钟,计算每分钟脉搏次数。小动物的脉搏不易摸测,可直接在动物左侧胸部用手触及心跳最明显处,计数一定时间,算出每分钟心跳次数,也可用听诊器或专用测量仪器进行测量。此外,在测量时应排除温度、湿度、动物
的终止麻醉,缺点是中、小型动物较适用,对大型动物如狗的吸入麻醉操作复杂,通常不用。具体方法是:使用乙醚麻醉兔及大小鼠时,可将动物放入玻璃麻醉箱内,把装有浸润乙醚棉球的小烧杯放入麻醉箱,然后观察动物。开始动物自主活动,不久动物出现异常兴奋,不停地挣扎,随后排出大小便。渐渐地动物由兴奋转为抑制,倒下不动,呼吸变慢。如动物四肢紧张度明显减低,角膜反射迟钝,皮肤痛觉消失,则表示动物已进入麻醉,可行手术和操作。在实验过程中应随时观察动物的变化,必要时把乙醚烧杯放在动物鼻部,以维持麻醉的时间与深度。2. 注射
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