参考文献 1.Chemistry and Biology of Hyaluronan, Edited by H.G. Garg and C.A. Hales, (2004). Publisher: Elsevier Oxford, UK
2.Hyaluronan in Cancer Biology, Edited by R.Stern, (2009) Publisher: Academic Press, San Diego, U.S.A
3.In 1936 Edwin Jelley sent a ‘Letter to the Editor’ of Nature, (138 1009 -1010), regarding the unusual behaviour of some cyanine dyes.
4.When dissolved in 5 M NaCl these dyes produce a third absorbance peak at a longer wave-length (650nm) whereas in deionised water only a double peak occurs at ~540 nm and ~570 nm.
5.The 650 nm peak in concentration dye solution induces aggregation of the dye molecules and has been described as a ‘J-aggregate’ named after Jelley.
6.Individual dye molecules may form a stacking format to produce a supra-
molecular complex as seen in peak 3, (Image on front cover of the Manual)
7.Further studies in the 1960s notably by Kay et.al. (J. Physical Chem. 68 1896 – 1906) found many biological polymers including proteins, DNA, polar lipids and glycosaminoglycans could induce the third peak when using high dye concentrations.
8.The dye was renamed ‘Stains-all’ by Dhlberg, Dingman and Peacock in1969
( J. Mol. Biol. 41, 139).