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上海希言科学仪器有限公司
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文献和实验the column into the injector. Finger tighten the column nut until it starts to grab the column, and then tighten the nut an additional 1/4 to 1/2 turn so that the column can’t be pulled from the fitting when gentle pressure is applied. 在进样口装柱
的话,用柱悬挂架。 3. Install the column nut and Vespel or graphite ferrule at each column end; pull the nut and ferrule down the tubing approximately 5 cm. 在柱两端按装螺母和Vespel或石墨垫。将螺母和垫圈顺着柱拉下约5厘米。 4. Score the column. Use a light touch to score the column
1. Remove 0.5 cm of tail into polypropylene microfuge tube (do not mince). (The tubes must have tight-fitting caps, so that there are no leaks in steps 3 and 7 below.) 2. Add 0.5 ml DNA digestion buffer with proteinase K added to 0.5 mg/ml final
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