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文献和实验BACs, PACs, P1s and Plasmid Isolation Protocol (standard protocol)
of at least 18,000 x g (a centrifugal force of 20,000 x g corresponds to 12,000 rpm in a Beckman JA-17 rotor, or 13,000 rpm in a Sorvall SS-34 rotor). 2. Sterile 1.5 ml Centrifuge Tubes 3. 10-15 ml Culture Tubes 实验步骤 1. Isolate
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
) for 15 minutes at 4°C to pellet cell debris and soft agar. Decant the supernatant and filter sterilize using a 0.45 μm filter. Store in a sterile screw-capped tube, tightly capped and protected from light, at 4°C.Prepare serial 1:10 dilutions from 10
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