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文献和实验Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
3) Puromycin, G418, and Ganciclovir Kill Curves A. Perform a kill curve for ganciclovir with cells stably expressing the Target ID Library and with puromycin or G418 for wild type cells. B. Plate 1.6 x 104 cells
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
riu m h ea d b lig h t in w h ea t b y exp ressio n o f A ra b id o p sis N P R l . Mol. Plant Microbe Interact.19, 123-129. 31. M a n o h a ra n , M . , D a h leen , L. S . , H o h n ,T. M . , N ea te, S . M . , Y u , X , H
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