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文献和实验functionalization of long?chain alkylamine controlled?pore glass (CPG) with a 3?hydroxypropyl?(2?cyanoethyl)thiophosphoryl linker and a dinucleoside phosphorotetrazolide leads to a uniquely engineered support for solid?phase synthesis. Unlike conventional
Preparation of Brain Membrane Fractions
.Neuroscience: 2713-2724. It should be followed when an enriched membrane preparation and postsynaptic densities are desired.Add 9 volumes of dissection buffer to the tissues and homogenize in a motor driven glass-FEP polymer homogenizer at 900 rpm (12–15
Preparation of Brain Membrane Fractions for Western Blot Analysis
volume of 15 ml 320 mM sucrose, add 9 volumes of water and immediately homogenize in glass-FEP polymer homogenizer (3 strokes at 1500 rpm). Proceed by adding the previously mentioned protease inhibitors. Incubate on ice for 15�30 min. Spin at 25,000
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