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文献和实验Long PCR Reagents and Guidelines
Efficient Long PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Following the results
Long-PCR Reagents and Guidelines
General Guidelines for Long-PCR Conditions and Enzyme Mixtures Efficient Long-PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading
Long-PCR Reagents and Guidelines
Long-PCR Reagents and Guidelines from George Church as Modified from Cheng et al. (1) General Guidelines for Long-PCR Conditions and Enzyme Mixtures ========== Following the results of Cheng et al. (1) we have had success using
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