- 询价
- Biotium
- 20465-100uL
- 美国
- 2025年12月07日
- ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot
- Goat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Alkaline Phosphatase Goat anti-rabbit IgG (H+L), 1 mg/mL 20465-100uL
- 靶点：
Rabbit IgG
- 浓度：
2 mg/mL
- 应用范围：
ELISA, Flow cytometry, IHC, IF (cells or tissue sections), Western blot
- 宿主：
Goat
- 保质期：
6个月
- 目录编号：
20465-100uL
- 库存：
99
- 供应商：
Biotium
- 标记物：
Alkaline Phosphatase
- 克隆性：
多克隆
- 保存条件：
-20°C
- 形态：
Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide, Lyophilized: PBS/15 mg/mL BSA/20 mg/mL trehalose after reconstitution
This is a goat anti-rabbit IgG (H+L) secondary antibody labeled with our superior CF® dyes and other labels.
- Available in 18 bright and photostable CF® dyes
- Alkaline phosphatase, APC, HRP, R-PE and biotin conjugates also available
- Suitable for western, immunofluorescence, and immunohistology in FFPE tissues
Note: Conjugates of blue fluorescent dyes like CF®350, CF®405S, and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Goat Anti-Rabbit IgG (H+L)
| Conjugation | Ex/Em | Size | Catalog No. |
|---|---|---|---|
| CF®350 | 347/448 nm | 50 uL (100 ug) | 20141-1 |
| 0.5 mL (1 mg) | 20141 | ||
| 1 mg (lyophilized) | 20141-1mg | ||
| CF®405S | 404/431 nm | 50 uL (100 ug) | 20082-1 |
| 0.5 mL (1 mg) | 20082 | ||
| 1 mg (lyophilized) | 20082-1mg | ||
| CF®405M | 408/452 nm | 50 uL (100 ug) | 20181-1 |
| 0.5 mL (1 mg) | 20181 | ||
| 1 mg (lyophilized) | 20181-1mg | ||
| CF®405L | 395/545 nm | 50 uL (100 ug) | 20409-50uL |
| 0.5 mL (1 mg) | 20409-500uL | ||
| 1 mg (lyophilized) | 20409-1mg | ||
| CF®488A | 490/515 nm | 50 uL (100 ug) | 20012-1 |
| 0.5 mL (1 mg) | 20012 | ||
| 1 mg (lyophilized) | 20012-1mg | ||
| CF®514 | 516/548 nm | 50 uL (100 ug) | 20387-50uL |
| 0.5 mL (1 mg) | 20387-500uL | ||
| 1 mg (lyophilized) | 20387-1mg | ||
| CF®532 | 527/558 nm | 50 uL (100 ug) | 20366-50uL |
| 0.5 mL (1 mg) | 20366-500uL | ||
| 1 mg (lyophilized) | 20366-1mg | ||
| CF®543 | 541/560 nm | 50 uL (100 ug) | 20309-1 |
| 0.5 mL (1 mg) | 20309 | ||
| 1 mg (lyophilized) | 20309-1mg | ||
| CF®555 | 555/565 nm | 50 uL (100 ug) | 20033-1 |
| 0.5 mL (1 mg) | 20033 | ||
| 1 mg (lyophilized) | 20033-1mg | ||
| CF®568 | 562/583 nm | 50 uL (100 ug) | 20102-1 |
| 0.5 mL (1 mg) | 20102 | ||
| 1 mg (lyophilized) | 20102-1mg | ||
| CF®594 | 593/614 nm | 50 uL (100 ug) | 20112-1 |
| 0.5 mL (1 mg) | 20112 | ||
| 1 mg (lyophilized) | 20112-1mg | ||
| CF®633 | 630/650 nm | 50 uL (100 ug) | 20122-1 |
| 0.5 mL (1 mg) | 20122 | ||
| 1 mg (lyophilized) | 20122-1mg | ||
| CF®640R | 642/662 nm | 50 uL (100 ug) | 20202-1 |
| 0.5 mL (1 mg) | 20202 | ||
| 1 mg (lyophilized) | 20202-1mg | ||
| CF®647 | 650/665 nm | 50 uL (100 ug) | 20043-1 |
| 0.5 mL (1 mg) | 20043 | ||
| 1 mg (lyophilized) | 20043-1mg | ||
| CF®660C | 667/685 nm | 50 uL (100 ug) | 20053-1 |
| 0.5 mL (1 mg) | 20053 | ||
| 1 mg (lyophilized) | 20053-1mg | ||
| CF®660R | 663/682 nm | 50 uL (100 ug) | 20055-1 |
| 0.5 mL (1 mg) | 20055 | ||
| 1 mg (lyophilized) | 20055-1mg | ||
| CF®750 | 755/777 nm | 50 uL (100 ug) | 20073-1 |
| 0.5 mL (1 mg) | 20073 | ||
| 1 mg (lyophilized) | 20073-1mg | ||
| CF®790 | 784/806 nm | 50 uL (100 ug) | 20379-50uL |
| R-PE | 496, 546, 565/578 nm | 1 mL (500 ug) | 20353-1mL |
| 200 uL (100 ug) | 20353-200uL | ||
| APC | 650/660 nm | 100 uL (50 ug) | 20412-100uL |
| 0.5 mL (250 ug) | 20412-500uL | ||
| Biotin | N/A | 50 uL (100 ug) | 20185 |
| 0.5 mL (1 mg) | 20185-1 | ||
| 1 mg (lyophilized) | 20185-1mg | ||
| HRP | N/A | 100 uL (100 ug) | 20402-100uL |
| 1 mL (1 mg) | 20402-1mL | ||
| 1 mg (lyophilized) | 20402-1mg | ||
| AP | N/A | 100 uL (100 ug) | 20465-100uL |
| 1 mL (1 mg) | 20465-1mL |
View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.
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文献和实验the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody preparation (serum, tissue culture supernatant, or ascites).Apply Binding buffer again ~ 200 ml.Apply Elution buffer ~ 100 ml.Collect 3 ml
g Stir O/N in cold room. Pour into plastic bottles. Spin at 4 o C, 7,500 rpm for 20 min in JA-10 rotor. Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer . Discard Sup. Resuspend pellets in 10 ml
的蛋白质样品(1-100??g)也能分离得很好，分辨率高使血清蛋白质可获得近20多个区带。 (二)试剂和器材 1.测试样品 血清蛋白、脱盐后的IgG、纯化后的IgG。 2.试剂 (1)制备分离胶、浓缩胶有关试剂 ① 凝胶缓冲液：称取1mol/L HCl 48ml，Tris(三羟基氨基甲烷)36.6g，TEMED(N，N，N`，N`-四甲基乙二胺)0.23ml，加重蒸水至80ml使其溶解，调pH8.9，然后加重蒸馏水定容至100
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