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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见说明书
- 保质期:
>1年
- 英文名:
Roscovitine Free Base
- 库存:
期货2-3周
- 供应商:
上海经科化学科技有限公司
- CAS号:
186692-46-6
- 规格:
10mg
供应商:上海经科化学科技有限公司
服务热线:400-0199-638
QQ:472482400(上海经科)
微信号:shjkchem
活动:消费积分可换充值卡!
本试剂(Roscovitine)
仅供科研实验使用,不得用于其他用途!
货 号:R-1234
名 称:Roscovitine
别 名:Roscovitine Free Base
C A S :186692-46-6
分子量:354.45
分子式:C19H26N6O
纯 度:HPLC/TLC:>99%
说 明:Storage: Store at or below -20 ºC. Solubility: Prepare initial stock solutions in DMSO (soluble at 200 mg/mL) or ethanol (soluble at 200 mg/mL) for in vitro use; solubility in water is low. Appearance: White to off-white powder. Disposal: A.
文 献:Roscovitine is a potent inhibitor of p34cdc2 (IC50 = 0.2 µM). Also inhibits p33cdk2 and p33cdk5. Havlicek, L., et al. "Cytokinin-derived cyclin-dependent kinase inhibitors: synthesis and cdc2 inhibitory activity of olomoucine and related compounds." J. Med. Chem. 40: 408-412 (1997).
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文献和实验Production of Cloned Calves Using Roscovitine-Treated Adult Somatic Cells as Donors
as donors to produce cloned animals. Recently, we have developed a novel and effective method using roscovitine to synchronize adult bovine granulosa cells in the G0 /G1 cell cycle stage. The resulting fetal and calf survival after transfer of cloned embryos
Detection of Apoptosis in Ovarian Cells In Vitro and In Vivo Using the Annexin V-Affinity Assay
of apoptosis during mammalian development ( 11 , 12 ). Fig. 1. Confocal scanning laser microscopy analysis of PA-1 ovary teratocarcinoma cells. Apoptosis was induced by treating the cells for 6 h with 50 μM roscovitine, a cyclin-dependent
不够,可选择继续培养再检测,这是该方法的一大优越之处. 研究特殊药物对细胞周期的影响(如roscovitine,一种抗癌药、周期蛋白激酶抑制剂),胰酶消化细胞、收集并铺于含药物的培养基上,37℃ 5%CO2 培养2天;除去之前的培养基换上含10%(v/v)alamarBlueTM 的新培养基,在预定的时间点检测其还原度。 (责任编辑:大汉昆仑王)
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