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兔抗光系统II Lhcb5蛋白多克隆抗体

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  • ¥4845
  • Agrisera
  • 货号AS01 009
  • 美国
  • 2025年07月10日
  • western blot (WB); immunohistochemistry (IHC)
  • 植物
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      兔抗光系统II Lhcb5蛋白多克隆抗体

    • 抗体英文名

      Lhcb5|CP26 chlorophyll a/b-binding protein of plant PSII

    • 浓度

      见说明书

    • 应用范围

      western blot (WB); immunohistochemistry (IHC)

    • 宿主

    • 适应物种

      植物

    • 标记物

    • 克隆性

      多抗/单行

    • 保存条件

      低温

    • 亚型

      IgG

    • 规格

      200 µl

    供应商:上海经科化学科技有限公司

    服务热线:400-0199-638


    QQ:472482400(上海经科)


    微信号:shjkchem

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    兔抗光系统II Lhcb5蛋白多克隆抗体介绍:

    货 号:AS01 009

    中文名称:兔抗光系统II Lhcb5蛋白多克隆抗体

    英文名称:Lhcb5|CP26 chlorophyll a/b-binding protein of plant PSII

    应用:western blot (WB); immunohistochemistry (IHC)

    规格:200 µl

    价格:4845元

    兔抗光系统II Lhcb5蛋白多克隆抗体简介:

    PRODUCT INFORMATION
    background

    Lhcb5 is one of the 3 minor highly conserved chlorophyll a/b-binding proteins associated with Photosystem II in plants and algae. As a part of the inner light-harvesting antenna it has been sugested to regulate (together with Lhcb4 and Lhcb6) the energy flow from the outer LHCII antenna to the PSII reaction center.

    immunogen

    BSA-conjugated synthetic peptide derived from Arabidosis thaliana Lhcb5 protein UniProt: Q9XF89, TAIR: AT4G10340 sequence. This sequence is highly conserved in Lhcb5 proteins from monocots, dicots and conifers but only partial conserved in Physcomitrella patens and Chlamydomonas reinhardtii.

    host

    rabbit

    clonality

    polyclonal,

    purity

    serum,

    format

    lyophilized

    quantity 200 µl
    reconstitution For reconstitution add 200 µl of sterile water
    storage

    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    tested applications

    western blot (WB); immunohistochemistry (IHC)

    related products AS01 009PRE Lhcb5 | CP26 chlorophyll a/b-binding protein of plant PSII, pre-immune serum​

    AS01 011 | A set of 10 plant anti-Lhca and anti-Lhcb antibodies

    AS01 011 Chlamydomonas | A set of anti-Lhc antibodies for Chlamydmonas

    Available antibodies against pigment-binding proteins- LHC

    recommended secondary antibody

    APPLICATION INFORMATION
    recommended dilution

    1 : 1000, detected with standard ECL (WB)

    expected | apparent MW

    30 | 25-26 kDa for Arabidopsis thaliana

    confirmed reactivity

    Arabidopsis thaliana, Hordeum vulgare, Drosera capensis, Oryza sativa, Pisum sativum, Spinacia oleracea, Zea mays

    predicted reactivity

    angiosperms (monocots and dicots), gymnosperms

    not reactive in

    no confirmed exceptions from predicted reactivity known in the moment

    additional information Protein is processed into mature form (Jansson 1999).
    selected references Pavlovič et al. (2016). A carnivorous sundew plant prefers protein over chitin as a source of nitrogen from its traps. Plant Physiol Biochem. 2016 Mar 5;104:11-16. doi: 10.1016/j.plaphy.2016.03.008
    Sun et al. (2014). Direct energy transfer from the major antenna to the photosystem II core complexes in the absence of minor antennae in liposomes. Biochim Biophys Acta. 2014 Nov 22. pii: S0005-2728(14)00650-1. doi: 10.1016/j.bbabio.2014.11.005
    Ido et al. (2014). Cross-Linking Evidence for Multiple Interactions of the PsbP and PsbQ Proteins in a Higher Plant Photosystem II Supercomplex. J Biol Chem. 2014 Jul 18;289(29):20150-7. doi: 0.1074/jbc.M114.574822. Epub 2014 Jun 9.
    Zhou et al. (2013) Mutation of the Light-Induced Yellow Leaf 1 Gene, Which Encodes a Geranylgeranyl Reductase, Affects Chlorophyll Biosynthesis and Light Sensitivity in Rice. PLoS ONE 8(9): e75299. doi:10.1371/journal.pone.0075299.

    application example

    5 µg protein of (1) chloroplasts, (2) thylakoids, and (3) Photosystem I of Arabidopsis thaliana together with (4) BBY (Photosystem II) -particles from Pisum sativum were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Lhcb5 (AS01 009, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL(GE Healthcare) using a Fuji LAS-3000 CCD (90s, standard sensitivity).

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