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兔抗β-1,3-葡聚糖酶多克隆抗体

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  • ¥5175
  • Agrisera
  • 货号AS07 208
  • 美国
  • 2025年07月10日
  • western blot (WB), immunolocalization (IL)
  • 植物
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 抗体名

      兔抗β-1,3-葡聚糖酶多克隆抗体

    • 抗体英文名

      PR-2|GLU I|class I beta-1,3-glucanase

    • 浓度

      见说明书

    • 应用范围

      western blot (WB), immunolocalization (IL)

    • 宿主

    • 适应物种

      植物

    • 标记物

    • 克隆性

      多抗/单行

    • 保存条件

      低温

    • 亚型

      IgG

    • 规格

      2 mg

    供应商:上海经科化学科技有限公司

    服务热线:400-0199-638


    QQ:472482400(上海经科)


    微信号:shjkchem

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    兔抗β-1,3-葡聚糖酶多克隆抗体介绍:

    货 号:AS07 208

    中文名称:兔抗β-1,3-葡聚糖酶多克隆抗体

    英文名称:PR-2|GLU I|class I beta-1,3-glucanase

    应用:western blot (WB), immunolocalization (IL)

    规格:2 mg

    价格:5175元

    兔抗β-1,3-葡聚糖酶多克隆抗体简介:

    PRODUCT INFORMATION
    background

    Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1,3-glucanase

    immunogen

    purified tobacco class I, basic ß-1,3-glucanase. Purified GLU I consists of a mixture of closely related polypeptides encoded by a family of GLU I genes comprising GLA B5APL3 derived from the sylvestris ancestor of tobacco, GLB P27666 derived from the tomentosiformis ancestor of tobacco and homeologous recombinants (Sperisen et al., 1991). Mature GLU I is processed from a pre-pro-polypeptide (Shinshi et al., 1988).

    host

    rabbit

    clonality

    polyclonal

    purity

    total IgG in PBS pH 7.4 (without Ca++)

    format

    lyophilized

    quantity 2 mg
    reconstitution For reconstitution add 100 µl of sterile water.
    storage

    store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

    tested applications

    western blot (WB), immunolocalization (IL)

    related products AS10 687 | PR-1 | Pathogenesis-related protein 1, rabbit antibodies

    AS12 2366 | PR-2 | pathogenesis-related protein 2, rabbit antibodies for Arabidopsis thaliana

    AS07 207 | PR-3 | CHN | class I chitinase, rabbit antibodies

    AS12 2369 | PR-4 | Pathogenesis-related protein 4, rabbit antibodies

    AS12 2373 | PR-5 | Pathogenesis-related protein 5, rabbit antibodies

    collection of antibodies to other proteins involved in a response to pathogen attack
    additional information

    for more details on immunolocalization, please referr to Keefe et al (1990). Plant 182: 43-51

    This antibody can be used as a marker of vacuolar contents Keefe et al. (1990). The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leave. Plant 182: 43-51.

    APPLICATION INFORMATION
    recommended dilution

    8 ug/ml with standard ECL (WB)

    expected | apparent MW

    37 | 33 kDa

    confirmed reactivity

    Nicotiana benthamian, Nicotiana clevilandii, Nicotiana glutinosa, Nicotiana tabacum, PhalenopsisSogo Yukidian cultivar V3, Populus sp., Solanum lycopersicum, Solanum tuberosum, Vitis vinifera

    predicted reactivity

    dicots

    not reactive in

    Arabidopsis thaliana

    additional information

    Important note: for blocking 5 % skim milk in PBS without Ca++ should be used.

    This antibody is purified by affinity chromarography on Portein G.

    selected references Wang et al. (2014). Elicitation of Hypersensitive Responses in Nicotiana glutinosa by the Suppressor of RNA Silencing Protein P0 from Poleroviruses. Mol Plant Pathol. 2014 Sep 4. doi: 10.1111/mpp.12201.
    Huey-wen et al. (2014). Harpin Protein, an Elicitor of Disease Resistance, Acts as a Growth Promoter in Phalaenopsis Orchids. Journal of Plant Growth Regulation May 2014.
    Munger et al. (2012). Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, Solanum tuberosum. BMC Plant Biol. 2012 Nov 1;12:198. doi: 10.1186/1471-2229-12-198.

    application example

    western blot detection of tobacco class I beta 1,3 glucanase

    Detection of tobacco tobacco class I ß 1,3 – glucanase in ng loaded per respective well using anti- tobacco class I ß 1,3 – glucanase antibodies. Primary antibodies have been used at 8 µg/ml.

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