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兔抗线粒体外膜蛋白孔蛋白 1多克隆抗体
VDAC1|voltage-dependent anion-selective channel protein 1
见说明书
western blot (WB), Blue-native (2D BN/SDS-PAGE),
兔
植物
无
多抗/单行
低温
IgG
200 µg
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兔抗线粒体外膜蛋白孔蛋白 1多克隆抗体介绍:
货 号:AS07 212
中文名称:兔抗线粒体外膜蛋白孔蛋白 1多克隆抗体
英文名称:VDAC1|voltage-dependent anion-selective channel protein 1
应用:western blot (WB), Blue-native (2D BN/SDS-PAGE),
规格:200 µg
价格:5175元
兔抗线粒体外膜蛋白孔蛋白 1多克隆抗体简介:
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APPLICATION INFORMATION | ||
recommended dilution | 1:5000 on 2-30 µg of protein/lane with standard ECL (WB), 1: 500 (IL) |
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expected | apparent MW | 29 kDa (for Arabidopsis thaliana) |
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confirmed reactivity | Arabidopsis thaliana, Beta vulgaris, Brassica oleracea var. botrytis, Oryza sativa, Papaver sp. pollen tubes (IL), Spinacia oleracea, Physcomitrella patens |
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predicted reactivity | Arabis alpina, Aundo donax, Brachypodium distachyon, Brassica campestris, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Citrus sinensis, Eutrema salsugineum, Glycine max, Glycine soja, Gossypium arboreum, Hoedum vulgare var. distichum, Jatropha curcas, Medicago truncatula, Mesembryanthemum crystallinum, Morus notabilis, Nicotiana tabacum, Phaseolus coccineus, Phaseolus vulgaris, Pisum sativum, Plantago major, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays |
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not reactive in | Chlamydomonas reinhardtii, Glycine max, Zea mays, diatoms, Saccharomyces cerevisiae |
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additional information | Amount of mitochondrial fraction detected by anti-VDAC1 antibody was from 2-10 µg. Immunolocalization method description and images are available here Blue-native (2D BN/SDS-PAGE) methodology is described in Piechota et al. 2010 |
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selected references | de Michele et al. (2016). Free-Flow Electrophoresis of Plasma Membrane Vesicles Enriched by Two-Phase Partitioning Enhances the Quality of the Proteome from Arabidopsis Seedlings. J Proteome Res. 2016 Mar 4;15(3):900-13. doi: 10.1021/acs.jproteome.5b00876. Epub 2016 Feb 4. Li et al. (2015). A Chaperone Function of NO CATALASE ACTIVITY1 Is Required to Maintain Catalase Activity and for Multiple Stress Responses in Arabidopsis. Plant Cell. 2015 Feb 19. pii: tpc.114.135095. Rurek et al. (2015). Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. Biochim Biophys Acta. 2015 Jan 21. pii: S0005-2728(15)00016-X. doi: 10.1016/j.bbabio.2015.01.005. Hsueh et al. (2014). The chloroplast outer envelope protein P39 in Arabidopsis thaliana belongs to the Omp85 protein family. Proteins. 2014 Nov 17. doi: 10.1002/prot.24725. Takahashi et al. (2014). Transport of rice cyclobutane pyrimidine dimer (CPD) photolyase into mitochondria relies on a targeting sequence located in its C-terminal internal region. Alcántar-Aguirre et al.(2013).ATP produced by oxidative phosphorylation is channeled toward hexokinase bound to mitochondrial porin (VDAC) in beetroots (Beta vulgaris). Planta, March 17. |
application example
10 μg of mitochondrial fraction from Arabidopsis thaliana and 25 μg of Arabidopsis thaliana leaf extract were separated on 10% gel and blotted on nitrocellulose membrane using wet transfer (0.22% CAPS, pH 11). Filters where blocked (1.5h) in 5% milk in TBST (1X TBS, 0,1% Tween 20), incubated with 1: 5000 anti-VDAC1 antibodies (2h in TBST) followed by incubation with 1: 10 000 secondary anti-rabbit (1h) HRP-coupled antibodies and visualized with standard ECL on Kodak autoradiography film for 15-60 s. Mitochondria were isolated as described by Urantowka et al. (Plant Mol Biol, 2005, 59:239-52). Mitochondrial pellets were suspended in 1X Laemmli buffer (5% beta-mercaptoetanol, 3.7% glycerol, 1.1% SDS, 23 mM Tris- HCl pH 6.8, 0.01% bromophenol blue), heated (95°C, 5 min.) and centrifuged (13 000rpm, 1 min.). Leaf extracts were prepared as described by Martinez-Garcia et al. (Plant J., 1999, 20:251-7).
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