western blot (WB), immunofluorescence (IF), immuno
宿主:
兔
适应物种:
植物
标记物:
无
克隆性:
多抗/单行
保存条件:
低温
亚型:
IgG
规格:
100 µg
供应商:上海经科化学科技有限公司
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QQ:472482400(上海经科)
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小鼠抗稗草内质网滞留信号HDEL单克隆抗体介绍:
货 号:AS10 683
中文名称:小鼠抗稗草内质网滞留信号HDEL单克隆抗体
英文名称:HDEL|endoplasmic reticulum retention signal
应用:western blot (WB), immunofluorescence (IF), immuno
规格:100 µg
价格:5565元
小鼠抗稗草内质网滞留信号HDEL单克隆抗体简介:
PRODUCT INFORMATION
background
HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
immunogen
synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
host
mouse
clonality
monoclonal
clone
IgG2B (clone 2E7)
format
liquid
quantity
100 µg
storage
store at -20°C for 1 year; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications
western blot (WB), immunofluorescence (IF), immunogold (IG)
protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml
APPLICATION INFORMATION
recommended dilution
1: 100 - 1. 1000 (WB), 1. 50- 1: 500 (IF)
expected | apparent MW
78 kDa
confirmed reactivity
Barnyard Grass (E. crus-galli), B. vulgaris, G. hirsutum, H. vulgare, Sorghum sp., V. radiata, S. cerevisiae, D. melanogaster
predicted reactivity
not analyzed
not reactive in
no confirmed exceptions from predicted reactivity known in the moment
additional information
antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7. For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992
selected references
Luo et al. (2006). GRP78/BiP is required for cell proliferation and protecting the inner cell mass from apoptosis during early mouse embryonic development. Mol Cell Biol. 26(15): 5688-5697. Napier et al. (1992). Immunological evidence that plants use both HDEL and KDEL for targeting proteins to the endoplasmic reticulum. J Cell Sci. 102: 261-271.