- ￥4214
- Agrisera
- 瑞典
- AS14 2783
- 2025年07月26日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海经科化学科技有限公司
- 抗体英文名：
SnRK2.2, SnRK2.3, SnRK2.6 | Ser/Thr-protein kinase SnRK
- 规格：
50ug
| Immunogen |
| KLH-conjugated synthetic peptide derived from Arabidopsis thaliana SnRK2.2 UniProt:Q39192 ,TAIR: At3g50500, SnRK2.3, UniProt: Q39193 TAIR: At5g66880, SnRK2.6 UniProt: Q940H6, TAIR: At4g33950 |
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Host Rabbit
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Clonality Polyclonal
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Purity Affinity purified serum in PBS, pH 7.4
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Format Lyophilized in PBS pH 7.4
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Quantity 50 µg
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Reconstitution For reconstitution add 50 µl of sterile water.
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Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Tested applications Western blot (WB)
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Recommended dilution 1 : 1000 (WB)
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Expected | apparent MW
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| 41 kDa |
名称：SnRK2.2, SnRK2.3, SnRK2.6 | Ser/Thr-protein kinase SnRK
货号：AS14 2783
品牌：Agrisera
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
, which may be used to specifically detect Ser, Thr, Ser/Thr, or Tyr residues phosphorylated by different protein kinase families. These antibodies are commonly used in immunoprecipitation and/or immunoblotting protocols to determine kinase?induced substrate
1 ul DNA x ul [[gamma]]-[32-P]-rATP 10 uCi T4 polynucleotide kinase 1 ul (3U/ul) 10 ul [[gamma]]-[32-P]-rATP (35020) ICN and T4 polynucleotide kinase (70031) from United States Biochemicals. 2. Incubate at 37degC
of the serine/threonine kinaseAkt, which will phosphorylate GSK-3 at specific serine residues(Ser21 in GSK-3a and Ser9 in GSK-3b), leading to the inactivationof GSK-3 kinase activity (10) . However, this phosphorylationand inactivation of GSK-3 by Akt
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