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文献和实验Intracellular Cytokine Staining Protocol
such as BSA or FCS can be added to the staining buffer. Optimal concentration of the fluorochrome-conjugated anti-cytokine antibodies has to be determined experimentally. To confirm specificity of the staining, it is common to block the directly-conjugated
Intracellular Cytokine Staining Protocol
than surface staining; therefore, extra protein such as BSA or FCS can be added to the staining buffer. Optimal concentration of the fluorochrome-conjugated anti-cytokine antibodies has to be determined experimentally. To confirm specificity of the staining
-Buffer® or LowCross-Buffer® STRONG as sample diluent, PlateBlock™ shows very good performance in serological ELISA tests, such as assays for neutralizing SARS-CoV-2 antibodies (s. also Fig. 5). The many benefits of animal-free solutions Animal
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