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- Neuromics
- KF17338-500
- 2025年07月15日
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文献和实验Blocking of unwanted non-specific staining
, which should be present in the development buffer. Blocking of endogenous fluorochromes. Blocking of endogenous fluorochromes is impossible. One may choose fluorochromes emitting in the UV range of spectrum, where endogenous autofluorescence of tissue is minimal.
Making Boiled Donkey (or whatever) Serum (BDS) for Blocking
Making Boiled Donkey (or whatever) Serum (BDS) for Blocking You want a final working concentration of 5% Serum. Order the serum from the same company and organism from which you buy secondary antibodies, Jackson Immunolabs
can often interfere with the blocking layer and cause faulty results. As you can see, the selection and performance evaluation of a surface blocker is a difficult but important task that cannot be solved with a one-buffer-fits-all solution if truly reliable assays
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