Anti-RFP (RABBIT) Antibody (Min X Hu Ms & Rt Serum Proteins) - 600-401-379
rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583.
Rabbit
Polyclonal
IgG
Target Details
DsRed - View All DsRed Products
RFP, mScarlet, rRFP, tdTomato
Recombinant Protein
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full-length amino acid sequence (234aa) derived from the mushroom anemone Discosoma.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins.
RFP Antibody Pre-adsorbed is designed for detecting RFP and its variants, tested across ELISA, Western blot, IF, IHC, and suitable for EM, FC, FISH, IP, and multiplex assays. It is applicable for ELISA in both sandwich and capture formats. Researchers should determine optimal titers for specific applications. Recommended starting dilutions are: ELISA 1:28,700 - 1:48,700; FC, IF, IHC 1:200 - 1:2,000; WB 1:1,000 - 1:5,000.
Formulation
1.03 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
step 5 minAntibody 15 min
PBS Wash 6 x 2 min
Protein A Gold 15 min
PBS Wash 6 x 2 min
Water Wash 5 x 1 min.
Blocking
Any "sticky" protein can be used to cover non-specific binding sites on the thawed sections
below.
Blocking step 5 minAntibody 15 min
PBS Wash 6 x 2 min
Protein A Gold 15 min
PBS Wash 6 x 2 min
Water Wash 5 x 1 min.
Blocking
Any "sticky" protein can be used to cover non-specific binding sites
), GSK-3b (dilution1:1,000; 1 h at RT), or b-actin (dilution 1:10,000; 1 h at RT).
5. Wash the membrane three times for 5 min each in TBS-T.
6. Incubate the membrane for 1 h at RT with 30 mL of TBS-Tcontaining anti-mouse or anti-rabbit IgG