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文献和实验Flow Cytometry Assay to Measure Internalization of GPCRs
engineered onto the N-terminus of the GPCR.The following protocol is optimized for internalization of FLAG-tagged beta 2-adrenergic receptor (B2AR) stably transfected into HEK293 cells.Protocol 1) Grow up receptors in 37degC incubator until confluent
Flow Cytometry Assay to Measure Internalization of GPCR's
protocol is optimized for internalization of FLAG-tagged beta 2-adrenergic receptor (B2AR) stably transfected into HEK293 cells. Protocol 1) Grow up receptors in 37degC incubator until confluent onto 6-cm dishes. 2) Fill dishes with 2 mL growth
Flow Cytometry Assay to Measure Internalization of GPCR
2-adrenergic receptor (B2AR) stably transfected into HEK293 cells. Protocol 1) Grow up receptors in 37degC incubator until confluent onto 6-cm dishes. 2) Fill dishes with 2 mL growth media with 30 mM HEPES, pH 7.4. Set aside a dish of 293/B2AR
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