诱导信号通道蛋白2(WISP2)检测试剂盒(酶联免疫吸附试验

诱导信号通道蛋白2(WISP2)检测试剂盒(酶联免疫吸附试验法)CCN5; CT58; CTGF-L; CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58 产品概述

诱导信号通道蛋白2(WISP2)检测试剂盒(酶联免疫吸附试验法)CCN5; CT58; CTGF-L; CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58（货号 SEG894Hu，英文名 WNT1）是Cloud-Clone提供的酶联免疫检测试剂盒，以酶联免疫吸附法（ELISA）检测Tissue、homogenates,、cell、lysates、and、other、biological、fluids.中的目标分子，规格48T/96T，适用于科研定量分析。

诱导信号通道蛋白2(WISP2)检测试剂盒(酶联免疫吸附试验法)CCN5; CT58; CTGF-L; CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58 检测原理

诱导信号通道蛋白2(WISP2)检测试剂盒(酶联免疫吸附试验法)CCN5; CT58; CTGF-L; CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58采用酶联免疫吸附法（ELISA）：固相载体上的捕获抗体与样本中待测物特异性结合，酶标二抗放大信号后显色，其 OD 值与浓度在一定区间内呈良好线性，据此完成定量。

诱导信号通道蛋白2(WISP2)检测试剂盒(酶联免疫吸附试验法)CCN5; CT58; CTGF-L; CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58 核心参数

WISP2 靶点背景与检测意义

WISP2（NCBI Gene ID 8839，官方基因名 CCN5）。This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes.

本试剂盒以 WISP2 为检测目标，适用于上述通路/功能相关的科研定量分析。

诱导信号通道蛋白2(WISP2)检测试剂盒(酶联免疫吸附试验法)CCN5; CT58; CTGF-L; CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58 适用样本与处理

本试剂盒适用于 Tissue、homogenates,、cell、lysates、and、other、biological、fluids.。样本采集后请按下列要点处理：

• 血清：采血后室温凝固 30 min，离心取上清，避免溶血；
• 血浆：使用合适抗凝剂采集，及时离心分离；
• 细胞培养上清：离心去除细胞碎片后取上清；
• 组织匀浆：按质量体积比加入裂解/匀浆缓冲液，离心取上清。

样本如不能立即检测，请分装后低温保存，避免反复冻融。

诱导信号通道蛋白2(WISP2)检测试剂盒(酶联免疫吸附试验法)CCN5; CT58; CTGF-L; CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58 操作流程

1. 试剂与样本平衡至室温，按随盒技术资料配制标准品、洗涤液及工作液；
2. 设置标准品孔、样本孔与空白孔，加入相应液体；
3. 孵育后洗板，加入酶标试剂再次孵育并洗涤；
4. 加入显色底物避光反应，加入终止液终止；
5. 于酶标仪读取吸光度（常规 450 nm），计算结果。

结果计算与标准曲线

以标准品浓度为横坐标、吸光度值为纵坐标绘制标准曲线（推荐四参数或对数拟合），将样本 OD 值代入求得浓度；超出曲线上限的样本请稀释后复测，结果乘以稀释倍数。

注意事项

• 本品仅供科研使用，不可用于临床诊断；
• 试剂请按 试剂盒 2-8℃ 避光保存，不得冷冻；复溶后的标准品请按要求分装并于 -20℃ 保存，避免反复冻融。；
• 不同批号组分请勿混用，洗板应充分以保证重复性；
• 显色时间需严格控制，终止后请及时读数。

质量控制与随附文件

产品出厂前经批间一致性、回收率与线性等质控项目检测，随盒提供质检报告（COA）与使用技术资料，检测范围与最低检出限以该批次 COA 标注为准。

诱导信号通道蛋白2(WISP2)检测试剂盒(酶联免疫吸附试验法)CCN5; CT58; CTGF-L; CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58 常见问题

Q1：诱导信号通道蛋白2(WISP2)检测试剂盒(酶联免疫吸附试验法)CCN5; CT58; CTGF-L; CCN family member 5; Connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58适合检测哪些样本？
A：适用于 Tissue、homogenates,、cell、lysates、and、other、biological、fluids.，其他样本类型建议先做预实验验证。

Q2：适用种属有哪些？
A：Homo sapiens (Human)，跨种属使用请先进行验证。

Q3：试剂盒如何保存、有效期多久？
A：试剂盒 2-8℃ 避光保存，不得冷冻；复溶后的标准品请按要求分装并于 -20℃ 保存，避免反复冻融。未开封 2-8℃ 条件下有效期 6 个月，具体以标签及质检报告（COA）为准。

参考文献（来源：PubMed）

[1] Grünberg JR et al. CCN5/WISP2 and metabolic diseases. Journal of cell communication and signaling. 2018. PMID:29247377

[2] Ferrand N et al. WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells. Cancers. 2022. PMID:35326638

[3] Alami T et al. Metabolic Effects of CCN5/WISP2 Gene Deficiency and Transgenic Overexpression in Mice. International journal of molecular sciences. 2021. PMID:34948212

靶点背景（来源：NCBI Gene）

WISP2（NCBI Gene ID 8839，官方基因名 CCN5，cellular communication network factor 5）。This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes.