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- QIGEN
- 德国
- 201913
- 2025年11月09日
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文献和实验Troubleshooting for PCR and multiplex PCR
, but keep MgCl2 concentration at 1.5-2mM Increase MgCl2 concentration up to 3-4.5 mM but keep dNTP concentration constant. Add adjuvants. Best, use BSA (0.1 to 0.8 µg/µL final concentration). You can also try 5% (v/v, final concentration) DMSO or glycerol
Protocol for competitive RT-PCR
, factor 2-2.5 between the standard amounts gives more accurate results (i.e.: 25 pg; 50 pg; 100 pg; 250 pg). In the following PCR 3-5 µl of cDNA, 1.5 units Taq DNA polymerase (Pharmacia), 200 µM of each dNTP, 250 nM of each primer and 1/10
Protocol for isolating DNA from embryo yolk sacs, or toes, or 2 mm tail for PCR
(not Tris base). The pH is about 5. Don't adjust the pH. For yolk sac/placenta/tail/toes 2. Add 40 µl (100 µl for tail/toe) 25 mM NaOH / 0.2 mM EDTA. 3. Place at 95°C for 20 minutes (in a thermal cycler) 4. Add 40 µl (100 µl for tail/toe) 40 mM Tris
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