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- 详细信息
- 技术资料
- 保存条件：
低温
- 库存：
现货
- 供应商：
上海善然生物科技有限公司
- CAS号：
1405-10-3
产品名称
Application
Neomycin Trisulfate is produced by Streptomyces containing a minimum of 85% neomycin B. It has been used as a selection agent for prokaryotic cells transformed using the neo selectable marker gene, and to study ototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts, and extraction of Nuclear Phosphatidylinositol 4,5-Bisphosphate-Interacting Proteins. It is recommended for use in cell culture applications at 50 mg/L.
Biochem/physiol Actions
作用方式：与引起错编的 30S 和 50S（有时为 50S）亚基结合；抑制蛋白质合成的开始和延长。
抗菌谱：革兰氏阴性和革兰氏阳性细菌。
Caution
Stock solutions should be filter sterilized and stored at 2-8°C, and are stable at 37°C for 5 days. A 1 mg/mL neomycin solution in .1 M phosphate buffer, pH 8.0 should be used within 14 days when stored at 0-5°C. Solutions should be protected from light or moisture.
包装
5, 25, 100 g in glass bottle
Preparation Note
Neomycin sulfate is soluble in H2O at 50 mg/mL, yielding a clear solution.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information
Application
Neomycin Trisulfate is produced by Streptomyces containing a minimum of 85% neomycin B. It has been used as a selection agent for prokaryotic cells transformed using the neo selectable marker gene, and to study ototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts, and extraction of Nuclear Phosphatidylinositol 4,5-Bisphosphate-Interacting Proteins. It is recommended for use in cell culture applications at 50 mg/L.
Biochem/physiol Actions
作用方式：与引起错编的 30S 和 50S（有时为 50S）亚基结合；抑制蛋白质合成的开始和延长。
抗菌谱：革兰氏阴性和革兰氏阳性细菌。
Caution
Stock solutions should be filter sterilized and stored at 2-8°C, and are stable at 37°C for 5 days. A 1 mg/mL neomycin solution in .1 M phosphate buffer, pH 8.0 should be used within 14 days when stored at 0-5°C. Solutions should be protected from light or moisture.
包装
5, 25, 100 g in glass bottle
Preparation Note
Neomycin sulfate is soluble in H2O at 50 mg/mL, yielding a clear solution.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information
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