Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 2 (Smad2)
抗体名:
Smad同源物2(Smad2)多克隆抗体
Smad同源物2(Smad2)多克隆抗体
Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 2 (Smad2)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species
Homo sapiens (Human)
Product No.
XYC124Hu01
Clonality
Polyclonal
Host
Rabbit
Immunoglobulin Type
IgG
Purification
Affinity Chromatography
Applications
WB, ICC, IHC-P, IHC-F, ELISA
Concentration
200ug/ml
UOM
100ug
Conjugate
No Conjugate
Smad同源物2(Smad2)多克隆抗体ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against Smad2. It has been selected for its ability to recognize Smad2 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.
IHC Images
Used in DAB staining on fromalin fixed paraffin-embedded Heart tissue
Smad同源物2(Smad2)多克隆抗体QUALITY CONTROL
Content: The quality control contains recombinant Smad2 (Trp274~Ser467) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
Smad同源物2(Smad2)多克隆抗体STORAGE
Store at 4oC for frequent use. Stored at -20oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.