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- Cell Signaling Technology
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- 2026年01月17日
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文献和实验several assays for detecting several kinds of DNA damage (strand breaks, internal crosslinking, DNA/protein crosslinks) and repair activity following exposure to genotoxic agents. The methods include single?cell electrophoresis (comet assay), filter eluting, K
Control of Mitotic Entry After DNA Damage in Drosophila
the collection of embryos and larvae, irradiation with x-rays to damage DNA, and fixing and staining of tissues with an antibody to phosphorylated histone H3 to measure the mitotic index. These methods should be useful in identifying potential mutants
that need to be optimized, including the choice of antibody, the conditions for shearing chromatin using sonication and the analysis of immunoprecipitated DNA. The ChIP-IT Kit from Active Motif provides a complete solution to all potential problems associated with the ChIP
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