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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20度
- 克隆性:
多克隆
- 适应物种:
其他
- 应用范围:
WB,ELISA等
- 抗体英文名:
anti-Digoxigenin
- 抗体名:
anti-Digoxigenin
- 规格:
0.1 mg
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
1. Add the following to a sterile microfuge tube on ice: -1 ug linearized plasmid DNA. -2 ul Dig RNA Labeling Mix, 10X concentrate -2 ul 10X transcription buffer -1-2 ul RNase inhibitor (DNase free) -add DEPC treated H2O up to 18 ul -2 ul RNA
In Situ Hybridization Using Digoxigenin Labeled Probes
dilution of Anti Digoxigenin-POD (Boehringer Cat. #1207 733). 1) Wash the slides in 1X PBS 2 times for 5 minutes each at room temperature 2) Wash for 2 minutes in 1X PBS/0.1 % Triton-X-100. 3) Rinse in 1X PBS 4) Place the slides horizontally
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