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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
请参考说明书
- 保质期:
请参考说明书
- 英文名:
FIX&PERM® Solution B (Perm)
- 库存:
大量
- 供应商:
欣博盛
- 规格:
1 x 100ml
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文献和实验b-galactosidase Solution Assay
b-galactosidase Solution Assay Reference: Simon and Lis (1987) NAR 15 : 2971-2988. Solutions CPRG Assay Buffer 50 mM KPO4 pH 7.5 5 ml 1M KPO4 pH 7.5 1 mM MgCl2 0.1 ml 1M MgCl 2 up to 100 ml with Q
b-galactosidase Solution Assay
of 0.25 M Tris HCl pH 8.0. • Freeze thaw the cells 3 times by alternating between dry ice and 37°. • Spin for 15 minutes at 4° and transfer the supernatant to a new tube and hold on ice. • Mix the following reaction solution per sample: 264
The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
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