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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Store at -20 deg C. After reconstitution store at -80 deg C. Avoid frequent freeze/thaw cycles
- 英文名：
Bet V 4 protein
- 库存：
大量
- 供应商：
欣博盛
- 规格：
1 mg
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文献和实验Partial proteolysis with Staph. aureus V8 protease
mM Tris-HCl, pH 6.8, and 1mM EDTA. This is in Tilo's box 3, in the east Forma, #1. Generally, if you aren't sure how much enzyme to use, try 10, 50 and 100 nanograms of V8 protease. If V8 doesn't work for your protein, try papain
Clustal V: Multiple Alignment of DNA and Protein Sequences
CLUSTAL is a package for performing fast and reliable automatic multiple alignment of many DNA or protein sequences. It was originally written for IBM -compatible microcomputers (1 ,2 ) and was later reorganized as a single program for VAX
7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
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