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文献和实验, and nuclear protein fractions that have been adapted for Arabidopsis . Two different protocols for the isolation of nuclei and nuclear extracts from Arabidopsis plants and suspension cells are described. Discovering that developmental mutant phenotypes
The ChIP (chromatin immunoprecipitation) method has emerged as a very powerful tool to study in vivo protein-DNA interactions
Dynabeads® Co-Immunoprecipitation Kit
, although the coupling reaction also works well with antibodies in storage buffers that include protein additives (e.g. BSA) and/or sodium azide (NaN3). This kit is not recommended for use with antibodies that have been stabilized in glycerol. 实验试剂
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